Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Troesken, E.R.; Adamska, M.; Arand, M.; Zarn, J.A.; Patten, C.; Voelkel, W.; Lutz, W.K.
    Comparison of lanosterol-14 alpha-demethylase (CYP51) of human and Candida albicans for inhibition by different antifungal azoles (2006), Toxicology, 228, 24-32.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 expression in Spodoptera frugiperda Sf9 cell membranes under control of the p10 promoter using the baculovirus transfection system Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.154 bifonazole IC50: 300 nM Candida albicans
1.14.14.154 bifonazole IC50: 0.0008 mM Homo sapiens
1.14.14.154 bitertanol IC50: 59 nM Candida albicans
1.14.14.154 bitertanol IC50: 0.0013 mM Homo sapiens
1.14.14.154 clotrimazole IC50: 91 nM Candida albicans
1.14.14.154 clotrimazole IC50: 0.0009 mM Homo sapiens
1.14.14.154 cyproconazole IC50: 100 nM Candida albicans
1.14.14.154 cyproconazole IC50: 0.0228 mM Homo sapiens
1.14.14.154 epoxiconazole IC50: 220 nM Candida albicans
1.14.14.154 epoxiconazole IC50: 0.0020 mM Homo sapiens
1.14.14.154 fadrozole IC50: 0.0322 mM, above Candida albicans
1.14.14.154 fadrozole IC50: 0.10 mM, above Homo sapiens
1.14.14.154 fluconazole IC50: 51 nM, above Candida albicans
1.14.14.154 fluconazole IC50: 0.030 mM, above Homo sapiens
1.14.14.154 flusilazole IC50: 85 nM Candida albicans
1.14.14.154 flusilazole IC50: 0.0034 mM Homo sapiens
1.14.14.154 hexaconazole IC50: 66 nM Candida albicans
1.14.14.154 hexaconazole IC50: 0.0156 mM Homo sapiens
1.14.14.154 imazalil IC50: 82 nM Candida albicans
1.14.14.154 imazalil IC50: 0.0361 mM Homo sapiens
1.14.14.154 itraconazole IC50: 39 nM Candida albicans
1.14.14.154 itraconazole IC50: 0.030 mM Homo sapiens
1.14.14.154 ketoconazole IC50: 64 nM Candida albicans
1.14.14.154 ketoconazole IC50: 0.0004 mM Homo sapiens
1.14.14.154 letrozole IC50: 0.0133 mM, above Candida albicans
1.14.14.154 letrozole IC50: 0.10 mM, above Homo sapiens
1.14.14.154 miconazole IC50: 72 nM Candida albicans
1.14.14.154 miconazole IC50: 0.00006 mM Homo sapiens
1.14.14.154 additional information inhibitory potency of fungicides, antifungal drugs, and cytostatic drugs, overview Candida albicans
1.14.14.154 additional information inhibitory potency of fungicides, antifungal drugs, and cytostatic drugs, overview Homo sapiens
1.14.14.154 myclobutanil IC50: 140 nM Candida albicans
1.14.14.154 myclobutanil IC50: 0.0290 mM Homo sapiens
1.14.14.154 penconazole IC50: 76 nM Candida albicans
1.14.14.154 penconazole IC50: 0.0193 mM Homo sapiens
1.14.14.154 Prochloraz IC50: 98 nM Candida albicans
1.14.14.154 Prochloraz IC50: 0.0050 mM Homo sapiens
1.14.14.154 Propiconazole IC50: 150 nM Candida albicans
1.14.14.154 Propiconazole IC50: 0.0083 mM Homo sapiens
1.14.14.154 tebuconazole IC50: 350 nM Candida albicans
1.14.14.154 tebuconazole IC50: 0.0036 mM Homo sapiens
1.14.14.154 triadimefon IC50: 130 nM Candida albicans
1.14.14.154 triadimefon IC50: 0.010 mM Homo sapiens
1.14.14.154 triadimenol IC50: 330 nM Candida albicans
1.14.14.154 triadimenol IC50: 0.0372 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.154 0.0017
-
 lanosterol pH 7.4, 37°C Homo sapiens
1.14.14.154 0.0074
-
 lanosterol pH 7.4, 37°C Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.154 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 ? production of follicular fluid-meiosis activating steroid by lanosterol demethylation ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Candida albicans
-
 ? production of follicular fluid-meiosis activating steroid by lanosterol demethylation ?
1.14.14.154 additional information Homo sapiens enzyme inhibition may result in endocrine disruption since follicular fluid-meiosis activating steroid, the direct product of lanosterol demethylation, is involved in the control of meiosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Candida albicans
-
-
-
1.14.14.154 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.154 additional information
-
-
 Homo sapiens
1.14.14.154 additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ? production of follicular fluid-meiosis activating steroid by lanosterol demethylation ?
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Candida albicans ? production of follicular fluid-meiosis activating steroid by lanosterol demethylation ?
1.14.14.154 additional information enzyme inhibition may result in endocrine disruption since follicular fluid-meiosis activating steroid, the direct product of lanosterol demethylation, is involved in the control of meiosis Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51
-
 Homo sapiens
1.14.14.154 CYP51
-
 Candida albicans
1.14.14.154 lanosterol-14alpha-demethylase
-
 Homo sapiens
1.14.14.154 lanosterol-14alpha-demethylase
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.154 37
-
 assay at Homo sapiens
1.14.14.154 37
-
 assay at Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.154 7.4
-
 assay at Homo sapiens
1.14.14.154 7.4
-
 assay at Candida albicans

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.14.154 0.0000091
-
 IC50: 91 nM Candida albicans clotrimazole
1.14.14.154 0.000039
-
 IC50: 39 nM Candida albicans itraconazole
1.14.14.154 0.000051
-
 IC50: 51 nM, above Candida albicans fluconazole
1.14.14.154 0.000059
-
 IC50: 59 nM Candida albicans bitertanol
1.14.14.154 0.00006
-
 IC50: 0.00006 mM Homo sapiens miconazole
1.14.14.154 0.000064
-
 IC50: 64 nM Candida albicans ketoconazole
1.14.14.154 0.000066
-
 IC50: 66 nM Candida albicans hexaconazole
1.14.14.154 0.000072
-
 IC50: 72 nM Candida albicans miconazole
1.14.14.154 0.000076
-
 IC50: 76 nM Candida albicans penconazole
1.14.14.154 0.000082
-
 IC50: 82 nM Candida albicans imazalil
1.14.14.154 0.000085
-
 IC50: 85 nM Candida albicans flusilazole
1.14.14.154 0.000098
-
 IC50: 98 nM Candida albicans Prochloraz
1.14.14.154 0.0001
-
 IC50: 100 nM Candida albicans cyproconazole
1.14.14.154 0.00013
-
 IC50: 130 nM Candida albicans triadimefon
1.14.14.154 0.00014
-
 IC50: 140 nM Candida albicans myclobutanil
1.14.14.154 0.00015
-
 IC50: 150 nM Candida albicans Propiconazole
1.14.14.154 0.00022
-
 IC50: 220 nM Candida albicans epoxiconazole
1.14.14.154 0.0003
-
 IC50: 300 nM Candida albicans bifonazole
1.14.14.154 0.00033
-
 IC50: 330 nM Candida albicans triadimenol
1.14.14.154 0.00035
-
 IC50: 350 nM Candida albicans tebuconazole
1.14.14.154 0.0004
-
 IC50: 0.0004 mM Homo sapiens ketoconazole
1.14.14.154 0.0008
-
 IC50: 0.0008 mM Homo sapiens bifonazole
1.14.14.154 0.0009
-
 IC50: 0.0009 mM Homo sapiens clotrimazole
1.14.14.154 0.0013
-
 IC50: 0.0013 mM Homo sapiens bitertanol
1.14.14.154 0.002
-
 IC50: 0.0020 mM Homo sapiens epoxiconazole
1.14.14.154 0.0034
-
 IC50: 0.0034 mM Homo sapiens flusilazole
1.14.14.154 0.0036
-
 IC50: 0.0036 mM Homo sapiens tebuconazole
1.14.14.154 0.005
-
 IC50: 0.0050 mM Homo sapiens Prochloraz
1.14.14.154 0.0083
-
 IC50: 0.0083 mM Homo sapiens Propiconazole
1.14.14.154 0.01
-
 IC50: 0.010 mM Homo sapiens triadimefon
1.14.14.154 0.0133
-
 IC50: 0.0133 mM, above Candida albicans letrozole
1.14.14.154 0.0156
-
 IC50: 0.0156 mM Homo sapiens hexaconazole
1.14.14.154 0.0193
-
 IC50: 0.0193 mM Homo sapiens penconazole
1.14.14.154 0.0228
-
 IC50: 0.0228 mM Homo sapiens cyproconazole
1.14.14.154 0.029
-
 IC50: 0.0290 mM Homo sapiens myclobutanil
1.14.14.154 0.03
-
 IC50: 0.030 mM Homo sapiens itraconazole
1.14.14.154 0.03
-
 IC50: 0.030 mM, above Homo sapiens fluconazole
1.14.14.154 0.0322
-
 IC50: 0.0322 mM, above Candida albicans fadrozole
1.14.14.154 0.0361
-
 IC50: 0.0361 mM Homo sapiens imazalil
1.14.14.154 0.0372
-
 IC50: 0.0372 mM Homo sapiens triadimenol
1.14.14.154 0.1
-
 IC50: 0.10 mM, above Homo sapiens fadrozole
1.14.14.154 0.1
-
 IC50: 0.10 mM, above Homo sapiens letrozole