EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.4.4 | crystals of E1b grown in the absence and presence of substrates at 22ΒΊC via the vapor-diffusion method, key tyrosine residue in the E1b active site, functions as a conformational switch to reduce the reactivity of the thiamin diphosphate cofactor, the tyrosine switch further remodels an E1b loop region to promote E1b binding to E2b | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.4.4 | additional information | hE1b-Tyr113Phe mutant, specifically affects the decarboxylation reaction, thiamine diphosphate appears hyperactive, is prone to paracatalytic inactivation | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.4 | alpha-keto-beta-methylvaleric acid + NADH | Homo sapiens | - |
? | - |
? | |
1.2.4.4 | alpha-ketoisocaproic acid + NADH | Homo sapiens | - |
? | - |
? | |
1.2.4.4 | alpha-ketoisovaleric acid + NADH | Homo sapiens | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.4 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.4.4 | alpha-keto-beta-methylvaleric acid + NADH | - |
Homo sapiens | ? | - |
? | |
1.2.4.4 | alpha-ketoisocaproic acid + NADH | - |
Homo sapiens | ? | - |
? | |
1.2.4.4 | alpha-ketoisovaleric acid + NADH | - |
Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.4.4 | BCKDC | - |
Homo sapiens |
1.2.4.4 | branched-chain alpha-ketoacid dehydrogenase complex | - |
Homo sapiens |
1.2.4.4 | E1b | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.4.4 | 0.1983 | - |
alpha-ketoisovaleric acid | wild-type | Homo sapiens | |
1.2.4.4 | 2.85 | - |
alpha-ketoisovaleric acid | hE1b-Tyr113Phe mutant | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.4.4 | thiamine diphosphate | - |
Homo sapiens |