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Literature summary extracted from

  • Martins, B.M.; Svetlitchnaia, T.; Dobbek, H.
    2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction (2005), Structure, 13, 817-824.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.61 purified enzyme, hanging drop vapour diffusion method, 50 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed with reservoir solution, containing 33–35% PEG 400, 200 mM ammonium tartrate, pH 6.5, in a 3:2 ratio with a resulting pH of 7.0, at aerobic conditions, 3 days at 17°C, under anaerobic conditions a reservoir solution containing 29–30% PEG 400, 200 mM ammonium tartrate, 5 mM Na-dithionite, pH 6.5, is mixed with protein reduced by 5 mM Na-dithionite in a ratio of 2:2 at 15°C, X-ray diffraction structure determination and analysis at 2.5 A resolution Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.61 Fe2+ the enzyme is a Rieske non-heme iron oxygenase, an active site Rieske-[2Fe-2S] center is involved in the enzyme complex, structure analysis of oxidized, reduced, and substrate bound states, overview Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.61 quinolin-2-ol + NADH + O2 Pseudomonas putida the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium quinolin-2,8-diol + NAD+ + H2O
-
 ?
1.14.13.61 quinolin-2-ol + NADH + O2 Pseudomonas putida 86 the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium quinolin-2,8-diol + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.61 Pseudomonas putida
-
-
-
1.14.13.61 Pseudomonas putida 86
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.61 native enzyme by ion exchange and hydrophobic interaction chromatography, and gel filtration Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.61 quinolin-2-ol + NADH + H+ + O2 = quinolin-2,8-diol + NAD+ + H2O active site modulation by Rieske-[2Fe-2S] center oxidation/reduction Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.61 quinolin-2-ol + NADH + O2 the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium Pseudomonas putida quinolin-2,8-diol + NAD+ + H2O
-
 ?
1.14.13.61 quinolin-2-ol + NADH + O2 the mononuclear Fe2+ ion and can open a pathway for dioxygen to bind in the substrate-containing active site, substrate binding structure, overview Pseudomonas putida quinolin-2,8-diol + NAD+ + H2O
-
 ?
1.14.13.61 quinolin-2-ol + NADH + O2 the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium Pseudomonas putida 86 quinolin-2,8-diol + NAD+ + H2O
-
 ?
1.14.13.61 quinolin-2-ol + NADH + O2 the mononuclear Fe2+ ion and can open a pathway for dioxygen to bind in the substrate-containing active site, substrate binding structure, overview Pseudomonas putida 86 quinolin-2,8-diol + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.61 More detailed enzyme structure analysis in oxidized, reduced, and substrate bound states, from cyrstal structure, overview Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.61 2-oxoquinoline 8-monooxygenase oxygenase
-
 Pseudomonas putida
1.14.13.61 OMO
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.61 NADH
-
 Pseudomonas putida