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Literature summary extracted from

  • Arhin, G.K.; Li, H.; Ullu, E.; Tschudi, C.
    A protein related to the vaccinia virus cap-specific methyltransferase VP39 is involved in cap 4 modification in Trypanosoma brucei (2006), RNA, 12, 53-62.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.57 additional information related to the vaccinia virus VP39 cap 2'-O-methyltransferase, may has a minor role in the biogenesis of the SL RNA cap 4 structure Trypanosoma brucei
2.1.1.57 additional information relatively low overall sequence conservation between vaccinia virus VP39 and TbMT57, has a major role in the biogenesis of the SL RNA cap 4 structure Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.296 the mt57-/- mutant cells are complemented with the triple MT57 mutant W62D/G63D/Q64D and with MT57 containing the K266A mutation, both mutants do not reestablish the +5 primer extension stop to wild-type levels Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.57 K266A proteins similar to that of the wild-type TbMT57-HA protein, defective in the biogenesis of the SL cap 4 structure Trypanosoma brucei
2.1.1.57 additional information trans-splicing utilization of the SL RNA is not detectably affected in mt57-/- cells, SS1 proteins similar to that of the wild-type TbMT57-HA protein, defective in the biogenesis of the SL cap 4 structure Trypanosoma brucei
2.1.1.296 K266A site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells Trypanosoma brucei
2.1.1.296 additional information genetic ablation of MT57 is compatible with cell viability and leads to the accumulation of SL RNA with a cap structure defective at positions +3 and +4.. Transsplicing utilization of the SL RNA is not detectably affected in mt57-/- cells, analysis of the SL cap structure in mt57-/- cells, overview Trypanosoma brucei
2.1.1.296 W62D/G63D/Q64D site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.57 nucleus
-
Trypanosoma brucei 5634
-
2.1.1.296 nucleus
-
Trypanosoma brucei 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.57 48000
-
-
Trypanosoma brucei
2.1.1.57 57000
-
-
Trypanosoma brucei
2.1.1.296 57000
-
x * 57000, SDS-PAGE Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.57 Trypanosoma brucei Q385S9
-
-
2.1.1.57 Trypanosoma brucei Q38DJ3
-
-
2.1.1.296 Trypanosoma brucei Q38DJ3
-
-

Subunits

EC Number Subunits Comment Organism
2.1.1.296 ? x * 57000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.1.1.57 cap-specific 2'-O-methyltransferase
-
Trypanosoma brucei
2.1.1.57 MT57
-
Trypanosoma brucei
2.1.1.57 TbMT48
-
Trypanosoma brucei
2.1.1.57 TbMT57
-
Trypanosoma brucei
2.1.1.296 MT57
-
Trypanosoma brucei
2.1.1.296 TbMT57
-
Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.1.1.296 evolution MT57 homologs are only found in trypanosomatid protozoa that have a cap 4 structure and in poxviruses, of which vaccinia virus is a prototype, TbMT48 or TbMT57 are two protein components of the cap 4 biosynthetic machinery Trypanosoma brucei
2.1.1.296 malfunction downregulation by RNAi or genetic ablation of TbMT57 result in the accumulation of SL RNA missing 2'-O-methyl groups at positions +3 and +4 and thus bearing a cap 2 rather than a cap 4. Genetic ablation of MT57 results in viable cells with no apparent defect in SL RNA transsplicing, suggesting that MT57 is not essential or that trypanosomes have developed alternate mechanisms to counteract the absence of this protein Trypanosoma brucei
2.1.1.296 physiological function the enzyme is involved in formation of the cap 4 structure, a cap structure of the SL RNA unique in eukaryotes with 4 nucleotides after the cap carrying a total of seven methyl groups. Modifications at the +3 and +4 positions are important for binding to the nuclear cap-binding complex, but MT57 is not essential. The Trypanosoma brucei cap binding complex can distinguish between a cap 4 and an m7G structure and it has a much higher affinity for the cap 4 substrate Trypanosoma brucei