EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.57 | additional information | related to the vaccinia virus VP39 cap 2'-O-methyltransferase, may has a minor role in the biogenesis of the SL RNA cap 4 structure | Trypanosoma brucei |
2.1.1.57 | additional information | relatively low overall sequence conservation between vaccinia virus VP39 and TbMT57, has a major role in the biogenesis of the SL RNA cap 4 structure | Trypanosoma brucei |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.296 | the mt57-/- mutant cells are complemented with the triple MT57 mutant W62D/G63D/Q64D and with MT57 containing the K266A mutation, both mutants do not reestablish the +5 primer extension stop to wild-type levels | Trypanosoma brucei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.57 | K266A | proteins similar to that of the wild-type TbMT57-HA protein, defective in the biogenesis of the SL cap 4 structure | Trypanosoma brucei |
2.1.1.57 | additional information | trans-splicing utilization of the SL RNA is not detectably affected in mt57-/- cells, SS1 proteins similar to that of the wild-type TbMT57-HA protein, defective in the biogenesis of the SL cap 4 structure | Trypanosoma brucei |
2.1.1.296 | K266A | site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells | Trypanosoma brucei |
2.1.1.296 | additional information | genetic ablation of MT57 is compatible with cell viability and leads to the accumulation of SL RNA with a cap structure defective at positions +3 and +4.. Transsplicing utilization of the SL RNA is not detectably affected in mt57-/- cells, analysis of the SL cap structure in mt57-/- cells, overview | Trypanosoma brucei |
2.1.1.296 | W62D/G63D/Q64D | site-directed mutagenesis, partly complements the enzyme defective mtant MT57-/- cells | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.1.57 | nucleus | - |
Trypanosoma brucei | 5634 | - |
2.1.1.296 | nucleus | - |
Trypanosoma brucei | 5634 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.57 | 48000 | - |
- |
Trypanosoma brucei |
2.1.1.57 | 57000 | - |
- |
Trypanosoma brucei |
2.1.1.296 | 57000 | - |
x * 57000, SDS-PAGE | Trypanosoma brucei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.57 | Trypanosoma brucei | Q385S9 | - |
- |
2.1.1.57 | Trypanosoma brucei | Q38DJ3 | - |
- |
2.1.1.296 | Trypanosoma brucei | Q38DJ3 | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.296 | ? | x * 57000, SDS-PAGE | Trypanosoma brucei |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.57 | cap-specific 2'-O-methyltransferase | - |
Trypanosoma brucei |
2.1.1.57 | MT57 | - |
Trypanosoma brucei |
2.1.1.57 | TbMT48 | - |
Trypanosoma brucei |
2.1.1.57 | TbMT57 | - |
Trypanosoma brucei |
2.1.1.296 | MT57 | - |
Trypanosoma brucei |
2.1.1.296 | TbMT57 | - |
Trypanosoma brucei |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.296 | evolution | MT57 homologs are only found in trypanosomatid protozoa that have a cap 4 structure and in poxviruses, of which vaccinia virus is a prototype, TbMT48 or TbMT57 are two protein components of the cap 4 biosynthetic machinery | Trypanosoma brucei |
2.1.1.296 | malfunction | downregulation by RNAi or genetic ablation of TbMT57 result in the accumulation of SL RNA missing 2'-O-methyl groups at positions +3 and +4 and thus bearing a cap 2 rather than a cap 4. Genetic ablation of MT57 results in viable cells with no apparent defect in SL RNA transsplicing, suggesting that MT57 is not essential or that trypanosomes have developed alternate mechanisms to counteract the absence of this protein | Trypanosoma brucei |
2.1.1.296 | physiological function | the enzyme is involved in formation of the cap 4 structure, a cap structure of the SL RNA unique in eukaryotes with 4 nucleotides after the cap carrying a total of seven methyl groups. Modifications at the +3 and +4 positions are important for binding to the nuclear cap-binding complex, but MT57 is not essential. The Trypanosoma brucei cap binding complex can distinguish between a cap 4 and an m7G structure and it has a much higher affinity for the cap 4 substrate | Trypanosoma brucei |