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Literature summary extracted from

  • Hall, M.P.; Ho, C.K.
    Characterization of a Trypanosoma brucei RNA cap (guanine N-7) methyltransferase (2006), RNA, 12, 488-497.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.56 analysis second Trypanosoma brucei RNA (guanine N-7) cap methyltransferase, named TbCgm1, TbCmt1 is homologous with TbCgm1, consisting of a C-terminal (guanine N-7) methyltransferase domain and an N-terminal guanylyltransferase domain, which contains signature motifs found in the nucleotidyl transferase superfamily Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.56 TbCmt1 inserted into an inducible T7 RNA polymerase-based pET vector and introduced into Escherichia coli BL21(DE3)Gold Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.56 D144A TbCmt1 mutant shows 5% of wild-type activity Trypanosoma brucei
2.1.1.56 D80A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
2.1.1.56 E258A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
2.1.1.56 F171A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
2.1.1.56 H174A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
2.1.1.56 K83A TbCmt1 mutant is unable to support methylation at levels of input protein sufficient to convert all the input substrate to the methylated form by wild-type TbCmt1, specific activity is less than 0.1% of the wild-type activity Trypanosoma brucei
2.1.1.56 V241A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
2.1.1.56 V242A TbCmt1 mutant displays near-wild-type activity Trypanosoma brucei
2.1.1.56 Y175A TbCmt1 mutant shows 3% of wild-type activity Trypanosoma brucei
2.1.1.56 Y246A TbCmt1 mutant shows 2% of wild-type activity Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.56 39000
-
 x * 39000, SDS-PAGE of His-tagged TbCmt1 Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.56 TbCmt1 protein purified from soluble extract by nickel-agarose, followed by phosphocellulose chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppN TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
2.1.1.56 S-adenosyl-L-methionine + GpppA TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppA
-
 ?
2.1.1.56 S-adenosyl-L-methionine + GpppG TbCmt1 Trypanosoma brucei S-adenosyl-L-homocysteine + m7GpppG
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.56 ? x * 39000, SDS-PAGE of His-tagged TbCmt1 Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.1.1.56 RNA cap (guanine N-7) methyltransferase
-
 Trypanosoma brucei
2.1.1.56 TbCgm1
-
 Trypanosoma brucei
2.1.1.56 TbCmt1
-
 Trypanosoma brucei