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Literature summary extracted from

  • Lai, M.C.; Wang, C.C.; Chuang, M.J.; Wu, Y.C.; Lee, Y.C.
    Effects of substrate and potassium on the betaine-synthesizing enzyme glycine sarcosine dimethylglycine N-methyltransferase from a halophilic methanoarchaeon Methanohalophilus portucalensis (2006), Res. Microbiol., 157, 948-955.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.162 S-adenosyl-L-homocysteine complete inhibition above 0.6 mM Methanohalophilus portucalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.162 0.4
-
 glycine pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.162 2.45
-
 N,N-dimethylglycine pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.162 3.14
-
 N-methylglycine pH 7.2, 37°C Methanohalophilus portucalensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.157 K+ highest activity at 0.8 M K+ Methanohalophilus portucalensis
2.1.1.162 K+ two different proteins eluted from chromatofocusing column are differently activated by addition of K+, the peak D protein exhibits low GSDMT activity only at K+ Methanohalophilus portucalensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.162 52000
-
 4 * 52000, SDS-PAGE, native mass of 240000 Da by native PAGE, different pI detected for the individual subunits Methanohalophilus portucalensis
2.1.1.162 240000
-
 native PAGE Methanohalophilus portucalensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine Methanohalophilus portucalensis FDF1 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N-methylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N-methylglycine Methanohalophilus portucalensis FDF1 synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + glycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N-methylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N-methylglycine Methanohalophilus portucalensis synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.157 Methanohalophilus portucalensis
-
 one peak showing SDMT activity eluted from chromatofocusing column
-
2.1.1.157 Methanohalophilus portucalensis FDF1
-
 one peak showing SDMT activity eluted from chromatofocusing column
-
2.1.1.162 Methanohalophilus portucalensis
-
-
-
2.1.1.162 Methanohalophilus portucalensis
-
 two different peaks showing GSDMT activity eluted from chromatofocusing column
-
2.1.1.162 Methanohalophilus portucalensis FDF1
-
-
-
2.1.1.162 Methanohalophilus portucalensis FDF1
-
 two different peaks showing GSDMT activity eluted from chromatofocusing column
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.162
-
 Methanohalophilus portucalensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.162 3 S-adenosyl-L-methionine + glycine = 3 S-adenosyl-L-homocysteine + betaine (overall reaction) Methanohalophilus portucalensis
a
2.1.1.162 S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine (1a) Methanohalophilus portucalensis
a
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine + H+ (1c) Methanohalophilus portucalensis
a
2.1.1.162 S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine (1b) Methanohalophilus portucalensis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.162 0.003
-
 N,N-dimethylglycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.162 0.0037
-
 glycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis
2.1.1.162 0.0046
-
 N-methylglycine as substrate, pH 7.2, 37°C Methanohalophilus portucalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.162 3 S-adenosyl-L-methionine + glycine (overall reaction) Methanohalophilus portucalensis 3 S-adenosyl-L-homocysteine + betaine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + glycine (1a) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + sarcosine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + glycine (1a) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + sarcosine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + glycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N-methylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine (1c) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + betaine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine (1c) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + betaine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N,N-dimethylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + N-methylglycine synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + sarcosine (1b) Methanohalophilus portucalensis S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?
2.1.1.162 S-adenosyl-L-methionine + sarcosine (1b) Methanohalophilus portucalensis FDF1 S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.162 tetramer 4 * 52000, SDS-PAGE, native mass of 240000 Da by native PAGE, different pI detected for the individual subunits Methanohalophilus portucalensis

Synonyms

EC Number Synonyms Comment Organism
2.1.1.157 SDMT
-
 Methanohalophilus portucalensis
2.1.1.162 GSDMT
-
 Methanohalophilus portucalensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.162 28
-
 glycine as subtrate Methanohalophilus portucalensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.162 Methanohalophilus portucalensis alpha-subunit, 2-D gel electrophoresis
-
 4.4
2.1.1.162 Methanohalophilus portucalensis beta-subunit, 2-D gel electrophoresis
-
 4.8
2.1.1.162 Methanohalophilus portucalensis gamma-subunit, 2-D gel electrophoresis
-
 5
2.1.1.162 Methanohalophilus portucalensis delta-subunit, 2-D gel electrophoresis
-
 5.2