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Literature summary extracted from

  • Abu-Abed, M.; Das, R.; Wang, L.; Melacini, G.
    Definition of an electrostatic relay switch critical for the cAMP-dependent activation of protein kinase A as revealed by the D170A mutant of RIalpha (2007), Proteins, 69, 112-124.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP
-
 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 D170A site-directed mutagenesis of the regulatory subunit RIalpha, the mutation selectively reduces the negative cooperativity between the cAMP- and C-recognition sites, i.e. the KD for the regulatory-catalytic subunit complex in the presence of cAMP is reduced by more than 12fold, without significantly compromising the high affinity of the regulatory subunit for both binding partners, conformational shifts upon mutation, and physiological implications of the dual functionality of the D170A mutant, overview Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.11 cytoplasm
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 Bos taurus 5737
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Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 additional information the regulatory subunit of PKA inhibits its kinase activity by shielding the catalytic subunit from physiological substrates, Asp170 not only plays a pivotal role in controlling the local conformation of the phosphate binding cassette, where cAMP docks, but also significantly affects the long-range cAMP-dependent interaction network that extends from the phosphate binding cassette to the three major sites of C-recognition Bos taurus ?
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 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 More the regulatory subunit of PKA inhibits its kinase activity by shielding the catalytic subunit from physiological substrates, interdependence between the Asp170 relay site and the regulatory-catalytic subunit interaction interface Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
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 Bos taurus
2.7.11.11 protein kinase A
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 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Bos taurus