EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.6.1.52 | hanging-drop vapor diffusion method at 22°C crystal. Structure of phosphoserine aminotransferase is determined at 1.2 and 1.5 A resolution at pH 8.5 and 4.6, respectively | Niallia circulans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.52 | 0.09 | - |
3-phosphonooxypyruvate | pH 9.0 | Niallia circulans | |
2.6.1.52 | 0.1 | - |
3-phosphonooxypyruvate | pH 7.0 | Niallia circulans | |
2.6.1.52 | 0.2 | - |
L-glutamate | pH 9.0 | Niallia circulans | |
2.6.1.52 | 1 | - |
L-glutamate | pH 7.0 | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.52 | Niallia circulans | Q59196 | ssp. alkalophilus, recombinant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.52 | recombinant | Niallia circulans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.52 | O-phospho-L-serine + 2-oxoglutarate | - |
Niallia circulans | 3-phosphonooxypyruvate + L-glutamate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.52 | BCIR PSAT | - |
Niallia circulans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | additional information | - |
release of pyridoxal-5'-phosphate and protein thermal denaturation occur simultaneously at pH 6.0 in contrast to pH 8.5 where denaturation preceds release of cofactors by approximately 3°C | Niallia circulans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 58 | - |
pH 8.5, Tm: 58.4°C | Niallia circulans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 9 | - |
phosphoserine synthesis | Niallia circulans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.52 | 8 | 9.5 | pH 8.0: about 50% of maximal activity, pH 9: about 60% of maximal activity, phosphoserine synthesis | Niallia circulans |