Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishijima, J.; Uchida, Y.; Kuroishi, C.; Tuzuki, C.; Takahashi, N.; Okazaki, N.; Yutani, K.; Miyano, M.
    Crystal structure of alanyl-tRNA synthetase editing-domain homolog (PH0574) from a hyperthermophile, Pyrococcus horikoshii OT3 at 1.45 A resolution (2006), Proteins, 62, 1133-1137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.7 gene PH0574, expression in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.7 purified recombinant wild-type and selenomethionine-labeld AlaRS editing-domain homolog, PH0574, 37.6 mg/ml protein, 27.5%w/v PEG 4000, and 100 mM MES-Na, pH 6.3, using a full-automatic protein crystallization and observation system, X-ray diffraction structure determination and analysis at 1.45 A resolution Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Mg2+
-
 Pyrococcus horikoshii
6.1.1.7 Zn2+ active site bound by His9, His13, His120, and Cys116, involved in the catalytic reaction Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 18152
-
 1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla Pyrococcus horikoshii
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla Pyrococcus horikoshii OT-3
-
 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Pyrococcus horikoshii O58307 strain OT3, gene PH0574
-
6.1.1.7 Pyrococcus horikoshii OT-3 O58307 strain OT3, gene PH0574
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7 recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Pyrococcus horikoshii AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview Pyrococcus horikoshii AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Pyrococcus horikoshii OT-3 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla AlaRS hydrolyzes mischarged tRNA by the catalytic zinc ion in the editing domain, modeling, overview Pyrococcus horikoshii OT-3 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.7 monomer 1 * 18152, crystal structure analysis, two-domain structure consisting of seven antiparallel beta-sheets and six helices Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.1.1.7 Alanyl-tRNA synthetase
-
 Pyrococcus horikoshii
6.1.1.7 AlaRS
-
 Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.7 ATP
-
 Pyrococcus horikoshii