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Literature summary extracted from

  • Stephenson, J.R.; Stacey, J.A.; Morgenthaler, J.B.; Friesen, J.A.; Lash, T.D.; Jones, M.A.
    Role of aspartate 400, arginine 262, and arginine 401 in the catalytic mechanism of human coproporphyrinogen oxidase (2007), Protein Sci., 16, 401-410.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 expressed in Escherichia coli BL21(DE3)RIL cells Homo sapiens
1.3.3.3 expressed in Escherichia coli strain BL21(DE3)RIL Homo sapiens
1.3.3.3 into vector pET21d Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.3 D400A impaired catalytic ability, with 0.047% of wild-type total product efficiency Homo sapiens
1.3.3.3 D400A the mutant produces no divinyl product, the Km value for coproporphyrinogen-III does not change significantly compared to the wild-type enzyme Homo sapiens
1.3.3.3 D400A the mutation impairs the catalytic ability of CPO relative to the wild type enzyme, not able to form divinyl products, but the Km value for coproporphyrinogen-III does not change significantly compared to the wild-type enzyme Homo sapiens
1.3.3.3 R262A impaired catalytic ability, with 0.00036% of wild-type total product efficiency Homo sapiens
1.3.3.3 R262A poor catalyst for the production of a divinyl product with a catalytic efficiency less than 0.01% compared to wild-type, including a 15fold higher Km for coproporphyrinogen-III Homo sapiens
1.3.3.3 R262A the mutant CPO is a poor catalyst for the production of a divinyl product, with a catalytic efficiency less than 0.01% compared to the wild type enzyme including a 15fold higher Km for coproporphyrinogen-III Homo sapiens
1.3.3.3 R401A impaired catalytic ability, with 44% of wild-type total product efficiency Homo sapiens
1.3.3.3 R401A the efficiency of divinyl product formation for mutant enzyme R401A is about 3% compared to wild type CPO, with a 3fold increase in the Km value for coproporphyrinogen-III Homo sapiens
1.3.3.3 R401A the efficiency of divinyl product formation is about 3% compared to the wild type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.00034
-
 Harderoporphyrinogen wild type enzyme Homo sapiens
1.3.3.3 0.00034
-
 Coproporphyrinogen-III wild-type, production to intermediate divinyl Homo sapiens
1.3.3.3 0.00056
-
 mesoporphyrinogen-VI wild type enzyme Homo sapiens
1.3.3.3 0.00056
-
 mesoporphyrinogen-VI wild type enzyme, at 37°C Homo sapiens
1.3.3.3 0.00056
-
 mesoporphyrinogen-VI wild-type, production to intermediate monovinyl Homo sapiens
1.3.3.3 0.00078
-
 Coproporphyrinogen-III mutant D400A Homo sapiens
1.3.3.3 0.00078
-
 Coproporphyrinogen-III mutant enzyme D400A Homo sapiens
1.3.3.3 0.00078
-
 Coproporphyrinogen-III mutant enzyme D400A, at 37°C Homo sapiens
1.3.3.3 0.00079
-
 Coproporphyrinogen-III wild-type Homo sapiens
1.3.3.3 0.00079
-
 Coproporphyrinogen-III wild type enzyme Homo sapiens
1.3.3.3 0.00079
-
 Coproporphyrinogen-III wild type enzyme, at 37°C Homo sapiens
1.3.3.3 0.00096
-
 Coproporphyrinogen-III mutant R401A, production to intermediate divinyl Homo sapiens
1.3.3.3 0.00096
-
 Harderoporphyrinogen mutant enzyme R401A Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant R401A, production to intermediate monovinyl Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant enzyme R401A Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant enzyme R401A, at 37°C Homo sapiens
1.3.3.3 0.0029
-
 Coproporphyrinogen-III mutant R401A Homo sapiens
1.3.3.3 0.0029
-
 Coproporphyrinogen-III mutant enzyme R401A Homo sapiens
1.3.3.3 0.0029
-
 Coproporphyrinogen-III mutant enzyme R401A, at 37°C Homo sapiens
1.3.3.3 0.0051
-
 Coproporphyrinogen-III mutant R262A, production to intermediate divinyl Homo sapiens
1.3.3.3 0.0051
-
 Harderoporphyrinogen mutant enzyme R262A Homo sapiens
1.3.3.3 0.0087
-
 Coproporphyrinogen-III mutant R262A Homo sapiens
1.3.3.3 0.0087
-
 Coproporphyrinogen-III mutant enzyme R262A Homo sapiens
1.3.3.3 0.0087
-
 Coproporphyrinogen-III mutant enzyme R262A, at 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.3 mitochondrion
-
 Homo sapiens 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Homo sapiens
-
-
-
1.3.3.3 Homo sapiens P36551 precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 Ni-NTA agarose metal affinity resin chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.3.3 coproporphyrinogen III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O CPO catalyzes two sequential oxidative decarboxylations of propionate moieties on coproporphyrinogen-III forming protoporphyrinogen-IX through the monovinyl intermediate harderoporphyrinogen Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+
-
 Homo sapiens protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+ Arg400, Arg262 and Arg401 are active amino acids critical for substrate binding and/or catalysis, Arg262 and 401 may coordinate carboxylate groups of coproporphyrinogen-III, while Asp400 may initiate deprotonation of substrate, resulting in oxidative decarboxylation Homo sapiens protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2 H+
-
 Homo sapiens harderoporphyrinogen + CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen-III + O2 + 2H+ CPO catalyzes the conversion of coproporphyrinogen-III to protoporphyrinogen-IX, a divinyl product, via two sequential oxidative decarboxylations of the A and B ring propionates Homo sapiens protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 harderoporphyrinogen + O2
-
 Homo sapiens protoporphyrinogen IX + CO2 + H2O2
-
 ?
1.3.3.3 harderoporphyrinogen + O2 + 2 H+
-
 Homo sapiens protoporphyrinogen-IX + CO2 + 2 H2O
-
 ?
1.3.3.3 harderoporphyrinogen + O2 + H+
-
 Homo sapiens protoporphyrinogen IX + CO2 + H2O2
-
 r
1.3.3.3 mesoporphyrinogen-VI + O2 + 2 H+
-
 Homo sapiens protoaetioporphyrin + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 mesoporphyrinogen-VI + O2 + 2 H+
-
 Homo sapiens protoaetioporphyrin + 2 CO2 + 2 H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.3.3.3 CPO
-
 Homo sapiens
1.3.3.3 CPO CPO is the sixth enzyme in the heme biosynthetic pathway Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.3 0.0000123
-
 Harderoporphyrinogen mutant enzyme R262A Homo sapiens
1.3.3.3 0.00002
-
 Coproporphyrinogen-III mutant D400A Homo sapiens
1.3.3.3 0.00002
-
 Coproporphyrinogen-III mutant enzyme D400A Homo sapiens
1.3.3.3 0.00002
-
 Coproporphyrinogen-III mutant enzyme D400A, at 37°C Homo sapiens
1.3.3.3 0.0004167
-
 Coproporphyrinogen-III mutant R262A Homo sapiens
1.3.3.3 0.00042
-
 Coproporphyrinogen-III mutant enzyme R262A, at 37°C Homo sapiens
1.3.3.3 0.00167
-
 Coproporphyrinogen-III mutant R401A, production to intermediate divinyl Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant R401A, production to intermediate monovinyl Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant enzyme R401A Homo sapiens
1.3.3.3 0.002
-
 mesoporphyrinogen-VI mutant enzyme R401A, at 37°C Homo sapiens
1.3.3.3 0.0063
-
 mesoporphyrinogen-VI wild type enzyme, at 37°C Homo sapiens
1.3.3.3 0.0063
-
 mesoporphyrinogen-VI wild-type, production to intermediate monovinyl Homo sapiens
1.3.3.3 0.00633
-
 mesoporphyrinogen-VI wild type enzyme Homo sapiens
1.3.3.3 0.017
-
 Harderoporphyrinogen mutant enzyme R401A Homo sapiens
1.3.3.3 0.02
-
 Coproporphyrinogen-III mutant R401A Homo sapiens
1.3.3.3 0.02
-
 Coproporphyrinogen-III mutant enzyme R401A Homo sapiens
1.3.3.3 0.02
-
 Coproporphyrinogen-III mutant enzyme R401A, at 37°C Homo sapiens
1.3.3.3 0.0267
-
 Coproporphyrinogen-III wild-type, production to intermediate divinyl Homo sapiens
1.3.3.3 0.027
-
 Harderoporphyrinogen wild type enzyme Homo sapiens
1.3.3.3 0.045
-
 Coproporphyrinogen-III wild-type Homo sapiens
1.3.3.3 0.045
-
 Coproporphyrinogen-III wild type enzyme Homo sapiens
1.3.3.3 0.045
-
 Coproporphyrinogen-III wild type enzyme, at 37°C Homo sapiens
1.3.3.3 0.42
-
 Coproporphyrinogen-III mutant enzyme R262A Homo sapiens