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Literature summary extracted from

  • Kawazoe, T.; Tsuge, H.; Pilone, M.S.; Fukui, K.
    Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring (2006), Protein Sci., 15, 2708-2717.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.3.3 hanging-drop vapor diffusion method, crystal structure of the enzyme in complex with the competitive inhibitor benzoate at a resolution of 2.5 A Homo sapiens

General Stability

EC Number General Stability Organism
1.4.3.3 homodimer is stable even in the apoprotein form Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.9
-
 D-Ala pH 8.3, 25°C Homo sapiens
1.4.3.3 1.7
-
 D-Pro pH 8.3, 25°C Homo sapiens
1.4.3.3 3.6
-
 D-Ser pH 8.3, 25°C Homo sapiens
1.4.3.3 140
-
 Gly pH 8.3, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 18
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-Ala + H2O + O2
-
 Homo sapiens pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-Pro + H2O + O2
-
 Homo sapiens 2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-Ser + H2O + O2
-
 Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 Gly + H2O + O2
-
 Homo sapiens acetate + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 DAO
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 0.6
-
 Gly pH 8.3, 25°C Homo sapiens
1.4.3.3 2.83
-
 D-Ser pH 8.3, 25°C Homo sapiens
1.4.3.3 5.5
-
 D-Ala pH 8.3, 25°C Homo sapiens
1.4.3.3 15
-
 D-Pro pH 8.3, 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD human enzyme weakly binds FAD and shows a significantly slower rate of flavin reduction compared to porcine enzyme Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.3 7
-
 benzoate
-
 Homo sapiens