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Literature summary extracted from

  • Stols, L.; Zhou, M.; Eschenfeldt, W.H.; Millard, C.S.; Abdullah, J.; Collart, F.R.; Kim, Y.; Donnelly, M.I.
    New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols (2007), Protein Expr. Purif., 53, 396-403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.5 genes scoA and scoB, encoding subunits of the heteromeric enzyme ScoAB, separate expression of the ScoAB subunits His-tagged ScoA and S-tagged ScoB Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.5 purified recombinant ScoA and ScoB, sitting drop vapour diffusion method, 69 mg/ml protein in 20 mM Hepes, pH 8.0, 200 mM NaCl and 1 mM DTT, mixing with reservoir solution containing 100 mM Tris, pH 8.5, 200 mM MgCl2, and 20% PEG 8000, at 16°C, X-ray diffraction structure determination and analysis at 2.89 A resolution Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate Bacillus subtilis
-
 succinate + acetoacetyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Bacillus subtilis
-
 genes scoA and scoB encode subunits of the heteromeric enzyme ScoAB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.5 recombinant ScoA and ScoB by metal affinity chromatography and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate
-
 Bacillus subtilis succinate + acetoacetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.8.3.5 More ScoAB subunits, separately expressed and mixed, do not interact effectively resulting in an inactive protein, overview Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.8.3.5 ScoAB
-
 Bacillus subtilis
2.8.3.5 succinyl-CoA:acetoacetate coenzyme A transferase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.5 30
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.5 9.1
-
 assay at Bacillus subtilis