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Literature summary extracted from

  • Mbewe, B.; Chibale, K.; McIntosh, D.B.
    Purification of human malaria parasite hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) using immobilized Reactive Red 120 (2007), Protein Expr. Purif., 52, 153-158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 EDTA
-
Plasmodium falciparum
2.4.2.8 additional information no inhibition by EGTA Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ activates Plasmodium falciparum
2.4.2.8 additional information no activation by Ca2+ Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum the enzyme is involved in the purine salvage pathway IMP + diphosphate
-
?
2.4.2.8 additional information Plasmodium falciparum the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 native enzyme by anion exchange and cation exchange chromatographies and affinity chromatography on immobilized Reactive Red 120, method development, overview, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 0.0000021 0.0000072 purified native enzyme Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum GMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is involved in the purine salvage pathway Plasmodium falciparum IMP + diphosphate
-
?
2.4.2.8 additional information the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own Plasmodium falciparum ?
-
?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme also performs the reaction of xanthine phosphoribosyltransferase, EC 2.4.2.22 Plasmodium falciparum XMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGXPRT
-
Plasmodium falciparum
2.4.2.8 hypoxanthine guanine xanthine phosphoribosyltransferase
-
Plasmodium falciparum
2.4.2.8 More cf. EC 2.4.2.22 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 22
-
assay at room temperature Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 8.5
-
assay at, substrates hypoxanthine and guanine Plasmodium falciparum