EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.8 | expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.8 | EDTA | - |
Plasmodium falciparum | |
2.4.2.8 | additional information | no inhibition by EGTA | Plasmodium falciparum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.8 | Mg2+ | activates | Plasmodium falciparum | |
2.4.2.8 | additional information | no activation by Ca2+ | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | the enzyme is involved in the purine salvage pathway | IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | Plasmodium falciparum | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.8 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.8 | native enzyme by anion exchange and cation exchange chromatographies and affinity chromatography on immobilized Reactive Red 120, method development, overview, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plasmodium falciparum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 0.0000021 | 0.0000072 | purified native enzyme | Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme is involved in the purine salvage pathway | Plasmodium falciparum | IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | Plasmodium falciparum | ? | - |
? | |
2.4.2.8 | xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme also performs the reaction of xanthine phosphoribosyltransferase, EC 2.4.2.22 | Plasmodium falciparum | XMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.8 | HGXPRT | - |
Plasmodium falciparum |
2.4.2.8 | hypoxanthine guanine xanthine phosphoribosyltransferase | - |
Plasmodium falciparum |
2.4.2.8 | More | cf. EC 2.4.2.22 | Plasmodium falciparum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 22 | - |
assay at room temperature | Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 8.5 | - |
assay at, substrates hypoxanthine and guanine | Plasmodium falciparum |