EC Number | Application | Comment | Organism |
---|---|---|---|
6.3.1.13 | drug development | MshC may represent a novel target for new classes of antituberculars | Mycolicibacterium smegmatis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.13 | gene mshC, construction of three N-terminal-MshC fusion proteins where N-terminal tags include the B1 domain of 1. streptococcal protein G to give GB1-MshC, 2. glutathione-S-transferase to give GST-MshC, and 3. maltose binding protein to give MBP-MshC, for expression in M. smegmatis, expression in enzyme-deficient mutant strain mc2155, i.e. I64 L205P, optimization of recombinant enzyme expression, overview | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | Mycolicibacterium smegmatis | key enzyme in the biosynthesis of mycothiol, pathway overview | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.13 | Mycolicibacterium smegmatis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.13 | recombinant GST-tagged MshC and recombinant maltose binding protein-MshC by affinity chromatography on glutathione and amylose resins, respectively | Mycolicibacterium smegmatis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.13 | additional information | - |
- |
Mycolicibacterium smegmatis |
6.3.1.13 | 0.009 | - |
purified recombinant GST-tagged MshC | Mycolicibacterium smegmatis |
6.3.1.13 | 0.025 | - |
purified recombinant maltose binding protein-MshC | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | - |
Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? | |
6.3.1.13 | 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP | key enzyme in the biosynthesis of mycothiol, pathway overview | Mycolicibacterium smegmatis | 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.13 | MshC | - |
Mycolicibacterium smegmatis |
6.3.1.13 | mycothiol ligase | - |
Mycolicibacterium smegmatis |