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Literature summary extracted from

  • Meng, Z.; Lou, Z.; Liu, Z.; Hui, D.; Bartlam, M.; Rao, Z.
    Purification, characterization, and crystallization of human pyrroline-5-carboxylate reductase (2006), Protein Expr. Purif., 49, 83-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 into a pET28a+ vector coding for a N-terminal His-tag for expression in Escherichia coli BL21DE3 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.2 the protein is crystallized by the hanging-drop vapor-diffusion method at 37°C and diffraction data are obtained to a resolution of 2.8 A Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 proline 0.1 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.141
-
 3,4-dehydro-L-proline
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.2 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 370000
-
 decamer, estimated by gel filtration and SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+ Homo sapiens
-
 L-proline + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Homo sapiens P32322
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 usung a Ni-NTA agarose, a Superdex-200 10/300 and a Resource Q column Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 hepatoma cell line full-length sequence is amplified from a hepatoma cell line cDNA library Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 1.6
-
 purification step: soluble cell fraction Homo sapiens
1.5.1.2 14
-
 purification step: Ni-NTA agarose Homo sapiens
1.5.1.2 14
-
 purification step: Resource Q Homo sapiens
1.5.1.2 21
-
 purification step: ammonium sulfate precipitation Homo sapiens
1.5.1.2 32
-
 purification step: Superdex 200 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 -80°C, 20 mM Caps, pH 9.4, 0.5 M NaCl, 6 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Homo sapiens L-proline + NADP+
-
 r
1.5.1.2 3,4-dehydro-L-proline + NAD+ activity assay Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.2 decamer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 P5CR
-
 Homo sapiens
1.5.1.2 pyrroline-5-carboxylate reductase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.2 25
-
 activity assay is performed at room temperature Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 90
-
 irreversible loss of activity at 90°C for 5 min Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 10
-
 3,4-dehydro-L-proline
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 9
-
 activity assay Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Homo sapiens
1.5.1.2 NADPH
-
 Homo sapiens