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Literature summary extracted from

  • Kasuya, F.; Tatsuki, T.; Ohta, M.; Kawai, Y.; Igarashi, K.
    Purification, characterization, and mass spectrometric sequencing of a medium chain acyl-CoA synthetase from mouse liver mitochondria and comparisons with the homologs of rat and bovine (2006), Protein Expr. Purif., 47, 405-414.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.2 diflunisal 0.001 mM, complete inhibition Bos taurus
6.2.1.2 diflunisal 0.001 mM, complete inhibition Mus musculus
6.2.1.2 enoxacin
-
 Bos taurus
6.2.1.2 enoxacin
-
 Mus musculus
6.2.1.2 Nalidixic acid 0.001 mM, complete inhibition Bos taurus
6.2.1.2 Nalidixic acid 0.001 mM, complete inhibition Mus musculus
6.2.1.2 ofloxacine
-
 Bos taurus
6.2.1.2 ofloxacine
-
 Mus musculus
6.2.1.2 salicylic acid 0.001 mM, complete inhibition Bos taurus
6.2.1.2 salicylic acid 0.001 mM, complete inhibition Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.2 mitochondrion
-
 Mus musculus 5739
-
6.2.1.2 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.2 65000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.2 Bos taurus
-
-
-
6.2.1.2 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.2 Sephadex G-25 gel filtration, DEAE-Sepharose column chromatography, and hydroxylapatite column chromatography Mus musculus
6.2.1.2 Sephadex G-25 gel filtration, DEAE-Sepharose column chromatography, and hydroxylapatite column chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.2 liver
-
 Mus musculus
-
6.2.1.2 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.2 0.023
-
 cell extract, with 0.001 mM hexanoic acid as a substrate in 0.2 M Tris-HCl buffer (pH 8.5) Mus musculus
6.2.1.2 4.14
-
 after 180fold purification, with 0.001 mM hexanoic acid as a substrate in 0.2 M Tris-HCl buffer (pH 8.5) Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.2 ATP + 1-naphthoic acid + CoA
-
 Mus musculus AMP + diphosphate + 1-naphthoyl-CoA
-
 ?
6.2.1.2 ATP + 1-naphthoic acid + CoA
-
 Bos taurus AMP + diphosphate + 1-naphthoyl-CoA
-
 ?
6.2.1.2 ATP + 1-naphthylacetic acid + CoA
-
 Mus musculus AMP + diphosphate + 1-naphthylacetoyl-CoA
-
 ?
6.2.1.2 ATP + 1-naphthylacetic acid + CoA
-
 Bos taurus AMP + diphosphate + 1-naphthylacetoyl-CoA
-
 ?
6.2.1.2 ATP + 3-methylbenzoate + CoA
-
 Mus musculus AMP + diphosphate + 3-methylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + 3-methylbenzoate + CoA
-
 Bos taurus AMP + diphosphate + 3-methylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + 4-methylbenzoate + CoA
-
 Mus musculus AMP + diphosphate + 4-methylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + 4-methylbenzoate + CoA
-
 Bos taurus AMP + diphosphate + 4-methylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + benzoate + CoA high activities are obtained with benzoate having methyl, pentyl, and methoxy groups in the para- or meta-positions of the benzene ring Mus musculus AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.2 ATP + benzoate + CoA high activities are obtained with benzoate having methyl, pentyl, and methoxy groups in the para- or meta-positions of the benzene ring Bos taurus AMP + diphosphate + benzoyl-CoA
-
 ?
6.2.1.2 ATP + decanoate + CoA
-
 Mus musculus AMP + diphosphate + decanoyl-CoA
-
 ?
6.2.1.2 ATP + decanoate + CoA
-
 Bos taurus AMP + diphosphate + decanoyl-CoA
-
 ?
6.2.1.2 ATP + hexanoate + CoA maximal activity on hexanoate Mus musculus AMP + diphosphate + hexanoyl-CoA
-
 ?
6.2.1.2 ATP + hexanoate + CoA maximal activity on hexanoate Bos taurus AMP + diphosphate + hexanoyl-CoA
-
 ?
6.2.1.2 ATP + ketoprofen + CoA less active on ketoprofen Mus musculus ADP + diphosphate + ?
-
 ?
6.2.1.2 ATP + ketoprofen + CoA less active on ketoprofen Bos taurus ADP + diphosphate + ?
-
 ?
6.2.1.2 ATP + m-methoxybenzoate + CoA
-
 Mus musculus AMP + diphosphate + m-methoxybenzoyl-CoA
-
 ?
6.2.1.2 ATP + m-methoxybenzoate + CoA
-
 Bos taurus AMP + diphosphate + m-methoxybenzoyl-CoA
-
 ?
6.2.1.2 ATP + m-pentylbenzoate + CoA
-
 Mus musculus AMP + diphosphate + m-pentylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + m-pentylbenzoate + CoA
-
 Bos taurus AMP + diphosphate + m-pentylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + octanoate + CoA
-
 Mus musculus AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.2 ATP + octanoate + CoA
-
 Bos taurus AMP + diphosphate + octanoyl-CoA
-
 ?
6.2.1.2 ATP + p-methoxybenzoate + CoA
-
 Mus musculus AMP + diphosphate + p-methoxybenzoyl-CoA
-
 ?
6.2.1.2 ATP + p-methoxybenzoate + CoA
-
 Bos taurus AMP + diphosphate + p-methoxybenzoyl-CoA
-
 ?
6.2.1.2 ATP + p-pentylbenzoate + CoA
-
 Mus musculus AMP + diphosphate + p-pentylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + p-pentylbenzoate + CoA
-
 Bos taurus AMP + diphosphate + p-pentylbenzoyl-CoA
-
 ?
6.2.1.2 ATP + valproate + CoA less active on valproate Mus musculus ADP + diphosphate + valproyl-CoA
-
 ?
6.2.1.2 ATP + valproate + CoA less active on valproate Bos taurus ADP + diphosphate + valproyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.2 medium chain acyl-CoA synthetase
-
 Mus musculus
6.2.1.2 medium chain acyl-CoA synthetase
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.2 0.0237
-
 enoxacin
-
 Mus musculus
6.2.1.2 0.0382
-
 ofloxacine
-
 Mus musculus