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Literature summary extracted from

  • Kang, J.H.; Park, Y.B.; Huh, T.L.; Lee, W.H.; Choi, M.S.; Kwon, O.S.
    High-level expression and characterization of the recombinant enzyme, and tissue distribution of human succinic semialdehyde dehydrogenase (2005), Protein Expr. Purif., 44, 16-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.24 expression in Escherichia coli as His-tag fusion protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.24 NADH NADH is a strong competitive inhibitor with respect to NAD+ and behaves as a non-competitive inhibitor with respect to succinate semialdehyde Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.24 6.3
-
 succinate semialdehyde 50 microM succinate semialdehyde, 3 mM NAD+, 0.1 M sodium diphosphate pH 8.5, 25°C Homo sapiens
1.2.1.24 125
-
 NAD+ 50 microM succinate semialdehyde, 3 mM NAD+, 0.1 M sodium diphosphate pH 8.5, 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.24 52000
-
 4 * 52000, gel-filtration Homo sapiens
1.2.1.24 52230
-
 calculated from sequence, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.24 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.24 Ni-affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.24 brain low mRNA expression compared to other tissues Homo sapiens
-
1.2.1.24 kidney mRNA expression Homo sapiens
-
1.2.1.24 liver highest mRNA expression Homo sapiens
-
1.2.1.24 skeletal muscle mRNA expression Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.24 succinate semialdehyde + NAD+ + H2O
-
 Homo sapiens succinate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.24 tetramer 4 * 52000, gel-filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.2.1.24 SSADH
-
 Homo sapiens
1.2.1.24 succinic semialdehyde dehydrogenase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.24 8.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.24 8 9 at least 80% of its optimal activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.24 0.1
-
 NADH succinate semialdehyde as varied substrate Homo sapiens
1.2.1.24 0.128
-
 NADH NAD+ as varied substrate Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.24 Homo sapiens computer prediction
-
 7.66