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Literature summary extracted from
- Purification and properties of 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase involved in microbial degradation of carbazole (2003), Protein Expr. Purif., 28, 182-189.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.7.1.8 | molecular biology | the deduced amino acid sequence of CarC shows 30.3, 31.3, and 31.8% identity with meta-cleavage compound hydrolases TodF, XylF, and DmpD, respectively, from other Pseudomonas | Pseudomonas sp. |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.7.1.8 | expression in Escherichia coli | Pseudomonas sp. |
| 3.7.1.13 | expression in Escherichia coli | Pseudomonas sp. |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.7.1.8 | S114A | ca. 40fold reduction in activity for the His tagged mutant hydrolase relative to similar lysates of His-tagged native CarC | Pseudomonas sp. |
| 3.7.1.13 | S114A | mutation in active-site serine. Approximately 40fold reduction in activity for the His-tagged mutant hydrolase relative to similar lysates of His-tagged native CarC | Pseudomonas sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.8 | 0.0046 | - |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid | at 25°C | Pseudomonas sp. |  |
| 3.7.1.13 | 0.0046 | - |
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid | pH 7.5, 25°C | Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.8 | 32231 | - |
2 * 32231, native enzyme, predicted from peptide sequence | Pseudomonas sp. |
| 3.7.1.8 | 55000 | - |
gel filtration | Pseudomonas sp. |
| 3.7.1.13 | 32231 | - |
x * 32231, calculated, x * 33000, SDS-PAGE | Pseudomonas sp. |
| 3.7.1.13 | 33000 | - |
x * 32231, calculated, x * 33000, SDS-PAGE | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.8 | Pseudomonas sp. | - |
LD2 | - |
| 3.7.1.8 | Pseudomonas sp. LD2 | - |
LD2 | - |
| 3.7.1.13 | Pseudomonas sp. | - |
- |
- |
| 3.7.1.13 | Pseudomonas sp. LD2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.8 | by metal affinity chromatography | Pseudomonas sp. |
| 3.7.1.13 | recombinant enzyme | Pseudomonas sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.8 | 2600 | - |
in 0.05 mM 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid, 50 mM Tris, pH 7.5 at 25°C | Pseudomonas sp. |
| 3.7.1.13 | 2600 | - |
pH 7.5, 25°C | Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.8 | 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. | 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? | |
| 3.7.1.8 | 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. LD2 | 2-aminobenzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? | |
| 3.7.1.13 | 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. | benzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? | |
| 3.7.1.13 | 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid + H2O | - |
Pseudomonas sp. LD2 | benzoic acid + 2-hydroxypenta-2,4-dienoic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.8 | homodimer | 2 * 32231, native enzyme, predicted from peptide sequence | Pseudomonas sp. |
| 3.7.1.13 | ? | x * 32231, calculated, x * 33000, SDS-PAGE | Pseudomonas sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.8 | 2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid hydrolase | - |
Pseudomonas sp. |
| 3.7.1.8 | CarC | - |
Pseudomonas sp. |
| 3.7.1.8 | HOPDA hydrolase | - |
Pseudomonas sp. |
| 3.7.1.13 | HOPDA hydrolase | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.8 | 58 | - |
- |
Pseudomonas sp. |
| 3.7.1.13 | 58 | - |
- |
Pseudomonas sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.8 | 1300 | - |
2-hydroxy-6-oxo-6-(2-aminophenyl)hexa-2,4-dienoic acid | - |
Pseudomonas sp. | |
| 3.7.1.13 | 1300 | - |
2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid | pH 7.5, 25°C | Pseudomonas sp. | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.8 | 7 | - |
- |
Pseudomonas sp. |
| 3.7.1.13 | 7 | - |
- |
Pseudomonas sp. |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.8 | 6.5 | 10.5 | - |
Pseudomonas sp. |
| 3.7.1.13 | 6.5 | - |
no activity | Pseudomonas sp. |
| 3.7.1.13 | 9 | - |
about 50% of maximal activity | Pseudomonas sp. |
| 3.7.1.13 | 10.5 | - |
no activity | Pseudomonas sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.8 | additional information | no cofactor requirement | Pseudomonas sp. | |
| 3.7.1.13 | additional information | no cofactor requirement | Pseudomonas sp. |
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