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Literature summary extracted from

  • Bae, J.; Lee, D.; Kim, D.; Cho, S.; Park, J.E.; Koh, S.; Kim, J.; Park, B.; Choi, Y.; Shin, H.; Hong, S.; Lee, D.
    Facile synthesis of glucose-1-phosphate from starch by Thermus caldophilus GK24 alpha-glucan phosphorylase (2005), Process Biochem., 40, 3707-3713.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.1 synthesis production of D-glucose-1-phosphate with yield of 47% w/w from a reaction containing 5% w/v soluble starch in 0.7 M potassium phosphate. Purification of D-glucose-1-phosphate by ethanol preciptitation Thermus caldophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.1
-
 Thermus caldophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.1 90000
-
 6 * 91677, calculated, 6 * 90000, SDS-PAGE Thermus caldophilus
2.4.1.1 91677
-
 6 * 91677, calculated, 6 * 90000, SDS-PAGE Thermus caldophilus
2.4.1.1 550000
-
 PAGE and gel filtration Thermus caldophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Thermus caldophilus
-
 overexpression of enzyme in Escherichia coli
-
2.4.1.1 Thermus caldophilus GK24
-
 overexpression of enzyme in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.1 recombinant protein expressed in Escherichia coli Thermus caldophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.1 15.5
-
-
 Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 amylopectin + phosphate
-
 Thermus caldophilus amylopectin + D-glucose-1-phosphate
-
 ?
2.4.1.1 amylopectin + phosphate
-
 Thermus caldophilus GK24 amylopectin + D-glucose-1-phosphate
-
 ?
2.4.1.1 amylose + phosphate
-
 Thermus caldophilus amylose + D-glucose-1-phosphate
-
 ?
2.4.1.1 amylose + phosphate
-
 Thermus caldophilus GK24 amylose + D-glucose-1-phosphate
-
 ?
2.4.1.1 starch + phosphate
-
 Thermus caldophilus starch + D-glucose-1-phosphate
-
 ?
2.4.1.1 starch + phosphate
-
 Thermus caldophilus GK24 starch + D-glucose-1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.1 hexamer 6 * 91677, calculated, 6 * 90000, SDS-PAGE Thermus caldophilus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 GlgP
-
 Thermus caldophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 70
-
-
 Thermus caldophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 7
-
 synthesis of glucose-1-phosphate Thermus caldophilus