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Literature summary extracted from

  • Kelly, M.J.; Ball, L.J.; Krieger, C.; Yu, Y.; Fischer, M.; Schiffmann, S.; Schmieder, P.; Kuhne, R.; Bermel, W.; Bacher, A.; Richter, G.; Oschkinat, H.
    The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site (2001), Proc. Natl. Acad. Sci. USA, 98, 13025-13030.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.99.12 drug development the enzyme is an attractive target for antibiotics, design of mechanism-based inhibitors Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.12
-
 Escherichia coli
4.1.99.12 expression in Escherichia coli Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.12 47000
-
-
 Methanocaldococcus jannaschii
4.1.99.12 47000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate Methanocaldococcus jannaschii the enzyme is involved in the pathway of riboflavin biosynthesis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate Escherichia coli the enzyme is involved in the pathway of riboflavin biosynthesis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.12 Escherichia coli P0A7J0
-
-
4.1.99.12 Methanocaldococcus jannaschii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.12 D-ribulose 5-phosphate
-
 Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate
-
 Escherichia coli formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate the enzyme is involved in the pathway of riboflavin biosynthesis Methanocaldococcus jannaschii formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate the enzyme is involved in the pathway of riboflavin biosynthesis Escherichia coli formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.12 dimer three-dimensional structure, structure-activity relationship study using NMR spectroscopy, residue-specific isotope labeling, and protein deuteration strategies, solution structure, overview Methanocaldococcus jannaschii
4.1.99.12 dimer three-dimensional structure, structure-activity relationship study using NMR spectroscopy, residue-specific isotope labeling, and protein deuteration strategies, solution structure, overview Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
4.1.99.12 DHBPS
-
 Methanocaldococcus jannaschii
4.1.99.12 DHBPS
-
 Escherichia coli