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Literature summary extracted from

  • Pillai, B.; Cherney, M.M.; Diaper, C.M.; Sutherland, A.; Blanchard, J.S.; Vederas, J.C.; James, M.N.
    Structural insights into stereochemical inversion by diaminopimelate epimerase: an antibacterial drug target (2006), Proc. Natl. Acad. Sci. USA, 103, 8668-8673.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.1.1.7 drug development bacterial racemase, including glutamate racemase and DAP epimerase, are potential targets for the development of new agents effective against organisms resistant to conventional antibiotics Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.1.7 co-crystals of the inhibitors LL- and DL-aziridino diaminopimelic acid with diaminopimelate epimerase from Haemophilus influenzae are grown at room temperature by the hanging-drop vapor-diffusion method. Crystals of both complexes are obtained in 2.8 M sodium acetate /0.1 M Hepes (pH 7.0) at a protein concentration of approx. 10 mg/ml in 25 mM Hepes, 5 mM DTT (pH 8.0) Haemophilus influenzae
5.1.1.7 crystal structures of diaminopimelate epimerase from Haemophilus influenzae with two different isomers of the irreversible inhibitor and substrate mimic aziridino diaminopimelic acid at 1.35- and 1.70-A resolution are analysed. These structures permit a detailed description of this pyridoxal 5’-phosphate-independent amino acid racemase active site and delineate the electrostatic interactions that control the exquisite substrate selectivity of DAP epimerase. Moreover, the active site shows how deprotonation of the substrates’nonacidic hydrogen at the alpha-carbon by a seemingly weakly basic cysteine residue is facilitated by interactions with two buried alpha-helices Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.7 DL-aziridino analogues of diaminoheptanedioate DL-aziridino diaminopimelic acid, irreversible inhibitor Haemophilus influenzae
5.1.1.7 LL-aziridino analogues of diaminoheptanedioate LL-aziridino diaminopimelic acid, irreversible inhibitor Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.7 Haemophilus influenzae P44859
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.7 LL-oxa-diaminopimelic acid
-
Haemophilus influenzae meso-oxa-diaminopimelic acid
-
?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.7 DAP epimerase
-
Haemophilus influenzae
5.1.1.7 diaminopimelate epimerase
-
Haemophilus influenzae