EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.1.7 | drug development | bacterial racemase, including glutamate racemase and DAP epimerase, are potential targets for the development of new agents effective against organisms resistant to conventional antibiotics | Haemophilus influenzae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.1.1.7 | co-crystals of the inhibitors LL- and DL-aziridino diaminopimelic acid with diaminopimelate epimerase from Haemophilus influenzae are grown at room temperature by the hanging-drop vapor-diffusion method. Crystals of both complexes are obtained in 2.8 M sodium acetate /0.1 M Hepes (pH 7.0) at a protein concentration of approx. 10 mg/ml in 25 mM Hepes, 5 mM DTT (pH 8.0) | Haemophilus influenzae |
5.1.1.7 | crystal structures of diaminopimelate epimerase from Haemophilus influenzae with two different isomers of the irreversible inhibitor and substrate mimic aziridino diaminopimelic acid at 1.35- and 1.70-A resolution are analysed. These structures permit a detailed description of this pyridoxal 5-phosphate-independent amino acid racemase active site and delineate the electrostatic interactions that control the exquisite substrate selectivity of DAP epimerase. Moreover, the active site shows how deprotonation of the substratesnonacidic hydrogen at the alpha-carbon by a seemingly weakly basic cysteine residue is facilitated by interactions with two buried alpha-helices | Haemophilus influenzae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.1.7 | DL-aziridino analogues of diaminoheptanedioate | DL-aziridino diaminopimelic acid, irreversible inhibitor | Haemophilus influenzae | |
5.1.1.7 | LL-aziridino analogues of diaminoheptanedioate | LL-aziridino diaminopimelic acid, irreversible inhibitor | Haemophilus influenzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.1.7 | Haemophilus influenzae | P44859 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.7 | LL-oxa-diaminopimelic acid | - |
Haemophilus influenzae | meso-oxa-diaminopimelic acid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.1.7 | DAP epimerase | - |
Haemophilus influenzae |
5.1.1.7 | diaminopimelate epimerase | - |
Haemophilus influenzae |