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Literature summary extracted from

  • Shi, J.; Dertouzos, J.; Gafni, A.; Steel, D.; Palfey, B.A.
    Single-molecule kinetics reveals signatures of half-sites reactivity in dihydroorotate dehydrogenase A catalysis (2006), Proc. Natl. Acad. Sci. USA, 103, 5775-5780.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.3.98.1 additional information significant heterogeneity in the catalytic behaviors of individual dimer molecules, very similar reaction rates in both the reductive and oxidative half-reactions for different DHODA dimers, single-molecule data provide strong evidence for half-sites reactivity, in which only one subunit reacts at a time Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.98.1 expressed in Escherichia coli strain SØ6645 Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
1.3.98.1 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.98.1 by ion-exchange chromatography Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.98.1 dihydroorotate + fumarate
-
 Lactococcus lactis orotate + reduced fumarate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.98.1 DHODA
-
 Lactococcus lactis