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Literature summary extracted from

  • Liavonchanka, A.; Hornung, E.; Feussner, I.; Rudolph, M.G.
    Structure and mechanism of the Propionibacterium acnes polyunsaturated fatty acid isomerase (2006), Proc. Natl. Acad. Sci. USA, 103, 2576-2581.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.B2 overexpression in Escherichia coli as a GST-fusion protein Cutibacterium acnes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.B2 six crystal structures in apo and product-bound forms, by single isomorphous replacement with anomalous scattering, SIRAS Cutibacterium acnes

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.B2 Cutibacterium acnes Q6A8X5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.B2 of the recombinant protein by glutathione sepharose chromatography Cutibacterium acnes

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.3.B2 (5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate double bond isomerization Cutibacterium acnes
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.B2 (6Z,9Z,12Z)-octadecatrienoic acid linolenic acid Cutibacterium acnes (6Z,10E,12Z)-octadecatrienoic acid
-
 ?
5.3.3.B2 (9Z,12Z)-octadecadienoic acid linoleic acid Cutibacterium acnes (10E,12Z)-octadecadienoic acid conjugated linoleic acid, double bound isomerization ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.B2 PAI polyenoic fatty acid isomerase from Propionibacterium acnes Cutibacterium acnes
5.3.3.B2 polyenoic fatty acid isomerase
-
 Cutibacterium acnes

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.B2 FAD
-
 Cutibacterium acnes