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Literature summary extracted from

  • Kawasaki, T.; Koita, H.; Nakatsubo, T.; Hasegawa, K.; Wakabayashi, K.; Takahashi, H.; Umemura, K.; Umezawa, T.; Shimamoto, K.
    Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice (2006), Proc. Natl. Acad. Sci. USA, 103, 230-235.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.44 OsRac1 interaction with OsRac1, one of the Rac/Rop family of small GTPases, lead to the enzymatic activation of OsCCR1 in vitro Oryza sativa
1.2.1.44 sphingolipid elicitor induction of expression Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 for the construction of a yeast two-hybrid assay prey vectors containing different fragments of OsCCR1 are made by using the pVP16 vector, for transient expression in rice protoplasts a OsCCR1-GFP construct is generated, full length OsCCR1 is cloned into a pET30 vector for expression in Escherichia coli cells Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.44 cytosol cytosolic localization of OsCCR1-GFP in rice protoplast Oryza sativa 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+ Oryza sativa
-
 cinnamoyl-CoA + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+
-
 Oryza sativa cinnamoyl-CoA + NADPH + H+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+ activity assay Oryza sativa coniferaldehyde + CoA + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 cinnamoyl-CoA reductase
-
 Oryza sativa
1.2.1.44 OsCCR1
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 30
-
 activity assay Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6.25
-
 activity assay Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADPH
-
 Oryza sativa