EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.50 | expression in Cos-1 cells | Homo sapiens |
1.1.1.213 | expression in Cos-1 cells | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one | competitive | Homo sapiens | |
1.1.1.50 | celecoxib | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.050 mM in fluorometric assay, in vitro IC50: 0.050 mM in fluorometric assay | Homo sapiens | |
1.1.1.50 | Flufenamic acid | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0040 mM in fluorometric assay, in vitro IC50: 0.00031 mM in fluorometric assay | Homo sapiens | |
1.1.1.50 | Ibuprofen | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.017 mM in fluorometric assay, in vitro IC50: 0.009 mM in fluorometric assay | Homo sapiens | |
1.1.1.50 | naproxen | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0094 mM in fluorometric assay, 0.016 mM in radiometric assay, in vitro IC50: 0.0027 mM in fluorometric assay | Homo sapiens | |
1.1.1.50 | ursodeoxycholate | natural inhibitor, in vivo IC50: 0.00024 mM in fluorometric assay, 0.00014 mM in radiometric assay, in vitro IC50: 0.000049 mM in fluorometric assay | Homo sapiens | |
1.1.1.213 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one | competitive | Homo sapiens | |
1.1.1.213 | celecoxib | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.050 mM in fluorometric assay, in vitro IC50: 0.050 mM in fluorometric assay | Homo sapiens | |
1.1.1.213 | Flufenamic acid | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0040 mM in fluorometric assay, in vitro IC50: 0.00031 mM in fluorometric assay | Homo sapiens | |
1.1.1.213 | Ibuprofen | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.017 mM in fluorometric assay, in vitro IC50: 0.009 mM in fluorometric assay | Homo sapiens | |
1.1.1.213 | naproxen | synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0094 mM in fluorometric assay, 0.016 mM in radiometric assay, in vitro IC50: 0.0027 mM in fluorometric assay | Homo sapiens | |
1.1.1.213 | ursodeoxycholate | natural inhibitor, in vivo IC50: 0.00024 mM in fluorometric assay, 0.00014 mM in radiometric assay, in vitro IC50: 0.000049 mM in fluorometric assay | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | 0.0013 | - |
5alpha-dihydrotestosterone | recombinant enzyme in intact COS-1 cells | Homo sapiens | |
1.1.1.50 | 0.003 | - |
9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one | recombinant enzyme in intact COS-1 cells | Homo sapiens | |
1.1.1.213 | 0.0013 | - |
5alpha-dihydrotestosterone | recombinant enzyme in intact COS-1 cells | Homo sapiens | |
1.1.1.213 | 0.003 | - |
9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one | recombinant enzyme in intact COS-1 cells | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH + H+ | Homo sapiens | inactivation of the potent androgen in human prostate, fluorometric activity measurement in intact cells, overview | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.50 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | Homo sapiens | competitive substrate, fluorometric activity measurement in intact cells, overview | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH + H+ | Homo sapiens | inactivation of the potent androgen in human prostate, fluorometric activity measurement in intact cells, overview | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.213 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | Homo sapiens | competitive substrate, fluorometric activity measurement in intact cells, overview | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Homo sapiens | - |
- |
- |
1.1.1.213 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.50 | prostate | - |
Homo sapiens | - |
1.1.1.213 | prostate gland | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH + H+ | inactivation of the potent androgen in human prostate, fluorometric activity measurement in intact cells, overview | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.50 | 5alpha-dihydrotestosterone + NADPH + H+ | i.e. DHT | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.50 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | competitive substrate, fluorometric activity measurement in intact cells, overview | Homo sapiens | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? | |
1.1.1.50 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | competitive substrate | Homo sapiens | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH + H+ | inactivation of the potent androgen in human prostate, fluorometric activity measurement in intact cells, overview | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.213 | 5alpha-dihydrotestosterone + NADPH + H+ | i.e. DHT | Homo sapiens | 5alpha-androstan-3alpha,17beta-diol + NADP+ | - |
? | |
1.1.1.213 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | competitive substrate, fluorometric activity measurement in intact cells, overview | Homo sapiens | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? | |
1.1.1.213 | 9-(phenylcarbonyl)-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADPH | competitive substrate | Homo sapiens | 9-[hydroxy(phenyl)methyl]-2,3,6,7-tetrahydro-1H,5H,11H-pyrano[2,3-f]pyrido[3,2,1-ij]quinolin-11-one + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.50 | AKR1C2 | - |
Homo sapiens |
1.1.1.50 | More | cf. EC 1.1.1.213, the enzyme belongs to the aldo-keto reductase, AKR, superfamily | Homo sapiens |
1.1.1.213 | AKR1C2 | - |
Homo sapiens |
1.1.1.213 | More | cf. EC 1.1.1.50, the enzyme belongs to the aldo-keto reductase, AKR, superfamily | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.50 | NADPH | - |
Homo sapiens | |
1.1.1.213 | NADPH | - |
Homo sapiens |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | 0.000049 | - |
natural inhibitor, in vivo IC50: 0.00024 mM in fluorometric assay, 0.00014 mM in radiometric assay, in vitro IC50: 0.000049 mM in fluorometric assay | Homo sapiens | ursodeoxycholate | |
1.1.1.50 | 0.00031 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0040 mM in fluorometric assay, in vitro IC50: 0.00031 mM in fluorometric assay | Homo sapiens | Flufenamic acid | |
1.1.1.50 | 0.0027 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0094 mM in fluorometric assay, 0.016 mM in radiometric assay, in vitro IC50: 0.0027 mM in fluorometric assay | Homo sapiens | naproxen | |
1.1.1.50 | 0.009 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.017 mM in fluorometric assay, in vitro IC50: 0.009 mM in fluorometric assay | Homo sapiens | Ibuprofen | |
1.1.1.50 | 0.05 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.050 mM in fluorometric assay, in vitro IC50: 0.050 mM in fluorometric assay | Homo sapiens | celecoxib | |
1.1.1.213 | 0.000049 | - |
natural inhibitor, in vivo IC50: 0.00024 mM in fluorometric assay, 0.00014 mM in radiometric assay, in vitro IC50: 0.000049 mM in fluorometric assay | Homo sapiens | ursodeoxycholate | |
1.1.1.213 | 0.00031 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0040 mM in fluorometric assay, in vitro IC50: 0.00031 mM in fluorometric assay | Homo sapiens | Flufenamic acid | |
1.1.1.213 | 0.0027 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.0094 mM in fluorometric assay, 0.016 mM in radiometric assay, in vitro IC50: 0.0027 mM in fluorometric assay | Homo sapiens | naproxen | |
1.1.1.213 | 0.009 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.017 mM in fluorometric assay, in vitro IC50: 0.009 mM in fluorometric assay | Homo sapiens | Ibuprofen | |
1.1.1.213 | 0.05 | - |
synthetic, nonsteroidal anti-inflammatory inhibitor, in vivo IC50: 0.050 mM in fluorometric assay, in vitro IC50: 0.050 mM in fluorometric assay | Homo sapiens | celecoxib |