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Literature summary extracted from
- Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase (2005), Proc. Natl. Acad. Sci. USA, 102, 8060-8065.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.145 | additional information | the enzyme expression is induced upon simulated insect attack using methyl jasmonate treatment of loblolly pine | Pinus taeda |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.145 | CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, de novo diterpenoid formation in metabolically engineered yeast, overview | Pinus taeda |
| 1.14.14.145 | CYP720B1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Pinus taeda |
| 1.14.14.145 | CYPA, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Pinus taeda |
| 1.14.14.145 | CYPA1, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Picea sitchensis |
| 1.14.14.145 | CYPA2, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Picea sitchensis |
| 1.14.14.145 | CYPB, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Pinus taeda |
| 1.14.14.145 | CYPC, functional cDNA screening and expression as FLAG-tagged protein in Saccharomyces cerevisiae, DNA and amino acid sequence determination and analysis | Pinus taeda |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.145 | additional information | - |
additional information | MichaelisMenten kinetics, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0005 | - |
abietadienal | pH 7.6, 30°C | Pinus taeda |  |
| 1.14.14.145 | 0.0005 | - |
abietadienal | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0006 | - |
dehydroabietadienal | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0006 | - |
isopimaradienal | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0006 | - |
dehydroabietadienal | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0006 | - |
isopimaradienal | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0008 | - |
abietadienol | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0008 | - |
abietadienol | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0015 | - |
isopimara-7,15-dienol | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0015 | - |
isopimaradienol | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0019 | - |
levopimaradienol | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0019 | - |
levopimaradienol | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
| 1.14.14.145 | 0.0053 | - |
dehydroabietadienol | pH 7.6, 30°C | Pinus taeda | |
| 1.14.14.145 | 0.0053 | - |
dehydroabietadienol | pH 7.6, 30°C, recombinant enzyme | Pinus taeda | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.145 | microsome | - |
Pinus taeda | - |
- |
| 1.14.14.145 | microsome | - |
Picea sitchensis | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.145 | abieta-7,13-dien-18-ol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Pinus taeda | - |
abieta-7,13-dien-18-oate + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | Pinus taeda | PtAO catalyzes an array of consecutive oxidation steps with several different diterpenol and diterpenal intermediates in loblolly pine diterpene resin acid biosynthesis | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | additional information | Pinus taeda | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense | ? | - |
? | |
| 1.14.14.145 | additional information | Picea sitchensis | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense | ? | - |
? | |
| 1.14.14.145 | additional information | Pinus taeda | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense, the enzyme catalyzes an array of consecutive oxidation steps with several different diterpenol and diterpenal intermediates in loblolly pine DRA biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.145 | Picea sitchensis | Q50EK0 | CYPA2; loblolly pine | - |
| 1.14.14.145 | Picea sitchensis | Q50EK1 | CYPA1; loblolly pine | - |
| 1.14.14.145 | Pinus taeda | - |
loblolly pine | - |
| 1.14.14.145 | Pinus taeda | Q50EK3 | CYPD; loblolly pine | - |
| 1.14.14.145 | Pinus taeda | Q50EK4 | CYPC; loblolly pine | - |
| 1.14.14.145 | Pinus taeda | Q50EK5 | CYPB; loblolly pine | - |
| 1.14.14.145 | Pinus taeda | Q50EK6 | - |
- |
| 1.14.14.145 | Pinus taeda | Q50EK6 | CYPA; loblolly pine | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.145 | additional information | CYP720B1 tissue profiling | Pinus taeda | - |
| 1.14.14.145 | stem | - |
Pinus taeda | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.145 | additional information | - |
substrate specificity, overview | Pinus taeda |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.145 | (13S)-pimara-7,15-dien-18-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | (13S)-pimara-7,15-dien-18-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | abieta-7,13-dien-18-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Pinus taeda | abieta-7,13-dien-18-oate + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 | PtAO catalyzes an array of consecutive oxidation steps with several different diterpenol and diterpenal intermediates in loblolly pine diterpene resin acid biosynthesis | Pinus taeda | abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abietadienal + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | abieta-7,13-dien-18-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | abietadienol + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | abietadienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | dehydroabietadienal + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | dehydroabietadienoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | dehydroabietadienol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | dehydroabietadienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | dehydroabietadienol + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | dehydroabietadienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | isopimara-7,15-dienal + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | isopimara-7,15-dien-18-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | isopimara-7,15-dienol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | isopimara-7,15-dienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | isopimara-7,15-dienol + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | isopimara-7,15-dienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | levopimaradienol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus taeda | levopimaradienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | levopimaradienol + [reduced NADPH-hemoprotein reductase] + O2 | oxidation at C-18 | Pinus taeda | levopimaradienal + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.145 | additional information | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense | Pinus taeda | ? | - |
? | |
| 1.14.14.145 | additional information | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense | Picea sitchensis | ? | - |
? | |
| 1.14.14.145 | additional information | the enzyme is involved in formation of diterpene resin acids, DRAs, in pine, and plays an important role in constitutive and induced oleoresin defense, the enzyme catalyzes an array of consecutive oxidation steps with several different diterpenol and diterpenal intermediates in loblolly pine DRA biosynthesis | Pinus taeda | ? | - |
? | |
| 1.14.14.145 | additional information | the enzyme CYP720B1 is a multifunctional, multisubstrate cytochrome P450 monooxygenase | Pinus taeda | ? | - |
? | |
| 1.14.14.145 | additional information | the recombinant enzyme catalyzed the oxidation of multiple diterpene alcohol and aldehydes. It is not able to oxidize abietadiene. No activity with neoabietadienol or neoabietadienal | Pinus taeda | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.145 | abietadienol/abietadienal oxidase | - |
Pinus taeda |
| 1.14.14.145 | CYP720B1 | - |
Pinus taeda |
| 1.14.14.145 | CyPA | - |
Pinus taeda |
| 1.14.14.145 | CYPA1 | - |
Picea sitchensis |
| 1.14.14.145 | CYPA2 | - |
Picea sitchensis |
| 1.14.14.145 | CyPB | - |
Pinus taeda |
| 1.14.14.145 | CYPC | - |
Pinus taeda |
| 1.14.14.145 | CYPD | - |
Pinus taeda |
| 1.14.14.145 | PtAO | - |
Pinus taeda |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.145 | 30 | - |
assay at | Pinus taeda |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.145 | 7.5 | 7.6 | - |
Pinus taeda |
| 1.14.14.145 | 7.6 | - |
assay at | Pinus taeda |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.145 | 7 | 8 | about 80% of maximal activity at pH 7.0 and 8.0 | Pinus taeda |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.145 | cytochrome P450 | - |
Pinus taeda |
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