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Literature summary extracted from

  • Siminszky, B.; Gavilano, L.; Bowen, S.W.; Dewey, R.E.
    Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase (2005), Proc. Natl. Acad. Sci. USA, 102, 14919-14924.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 CYP82E2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
1.14.14.1 CYP82E3, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
1.14.14.1 CYP82E4v1, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
1.14.14.1 CYP82E4v2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11 Nicotiana tabacum
1.14.14.B11 expression in Saccharomyces cerevisiae Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.1 additional information construction of transgenic plants overexpressing the enzyme, analysis of alkaloid spectrum, overview Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.1 microsome
-
 Nicotiana tabacum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.1 additional information Nicotiana tabacum the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco ?
-
 ?
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum
-
 nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine ?
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2 Nicotiana tabacum nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Nicotiana tabacum Q38Q84 CYP82E3
-
1.14.14.1 Nicotiana tabacum Q38Q85 CYP82E2
-
1.14.14.1 Nicotiana tabacum Q38Q86 CYP82E4v1
-
1.14.14.1 Nicotiana tabacum Q38Q87 CYP82E4v2; three CYP82E isozymes
-
1.14.14.B11 Nicotiana tabacum Q38Q86 CYP82E4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.1 leaf
-
 Nicotiana tabacum
-
1.14.14.B11 leaf cured leaf Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 additional information the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco Nicotiana tabacum ?
-
 ?
1.14.14.1 additional information the enzyme demethylates nicotine to nornicotine Nicotiana tabacum ?
-
 ?
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2
-
 Nicotiana tabacum nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP82E4v1 is responsible for the conversion of nicotine to nornicotine ?
1.14.14.B11 nicotine + [reduced NADPH-hemoprotein reductase] + O2 nornicotine is a secondary tobacco alkaloid that is produced by the N-demethylation of nicotine. Nornicotine production and accumulation in tobacco are undesirable because nornicotine serves as the precursor in the synthesis of the carcinogen N-nitrosonornicotine during the curing and processing of tobacco Nicotiana tabacum nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 CYP82E2
-
 Nicotiana tabacum
1.14.14.1 CYP82E3
-
 Nicotiana tabacum
1.14.14.1 CYP82E4v1
-
 Nicotiana tabacum
1.14.14.1 CYP82E4v2
-
 Nicotiana tabacum
1.14.14.1 cytochrome P450 monooxygenase
-
 Nicotiana tabacum
1.14.14.1 More the enzyme belongs to the CYP82E2 family Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.B11 cytochrome P450
-
 Nicotiana tabacum