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Literature summary extracted from
- Purification and characterization of a novel catechol 1,2-dioxygenase from Pseudomonas aeruginosa with benzoic acid as a carbon source (2006), Proc. Biochem., 41, 1594-1601.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.13.11.1 | Ag+ | 0.5 mM, complete inhibition | Pseudomonas aeruginosa |  |
| 1.13.11.1 | Cu2+ | 0.5 mM, 81% inhibition | Pseudomonas aeruginosa | |
| 1.13.11.1 | Mn2+ | 0.5 mM, 64% inhibition | Pseudomonas aeruginosa | |
| 1.13.11.1 | PCMB | 0.5 mM, complete inhibition | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.1 | 0.0032 | - |
3-methylcatechol | - |
Pseudomonas aeruginosa | |
| 1.13.11.1 | 0.0059 | - |
catechol | - |
Pseudomonas aeruginosa | |
| 1.13.11.1 | 0.013 | - |
4-methylcatechol | - |
Pseudomonas aeruginosa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.13.11.1 | intracellular | - |
Pseudomonas aeruginosa | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 22000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.1 | Pseudomonas aeruginosa | - |
TKU002 | - |
| 1.13.11.1 | Pseudomonas aeruginosa TKU002 | - |
TKU002 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.13.11.1 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 10 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.1 | 3-methylcatechol + O2 | 10% of the activity with catechol | Pseudomonas aeruginosa | 2-methyl-cis,cis-muconate | - |
? | |
| 1.13.11.1 | 3-methylcatechol + O2 | 10% of the activity with catechol | Pseudomonas aeruginosa TKU002 | 2-methyl-cis,cis-muconate | - |
? | |
| 1.13.11.1 | 4-chlorocatechol + O2 | 11% of the activity with catechol | Pseudomonas aeruginosa | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | 4-chlorocatechol + O2 | 11% of the activity with catechol | Pseudomonas aeruginosa TKU002 | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
| 1.13.11.1 | 4-methylcatechol + O2 | 43% of the activity with catechol | Pseudomonas aeruginosa | 3-methyl-cis,cis-muconate | - |
? | |
| 1.13.11.1 | 4-methylcatechol + O2 | 43% of the activity with catechol | Pseudomonas aeruginosa TKU002 | 3-methyl-cis,cis-muconate | - |
? | |
| 1.13.11.1 | catechol + O2 | - |
Pseudomonas aeruginosa | cis,cis-muconate | - |
? | |
| 1.13.11.1 | catechol + O2 | - |
Pseudomonas aeruginosa TKU002 | cis,cis-muconate | - |
? | |
| 1.13.11.1 | pyrogallol + O2 | 180% of the activity with catechol | Pseudomonas aeruginosa | alpha-hydroxymuconic acid | - |
? | |
| 1.13.11.1 | pyrogallol + O2 | 180% of the activity with catechol | Pseudomonas aeruginosa TKU002 | alpha-hydroxymuconic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.13.11.1 | monomer | 1 * 22000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 40 | - |
- |
Pseudomonas aeruginosa |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 20 | 50 | 20°C: about 30% of maximal activity, 50°C: about 20% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 40 | - |
1 h, stable up to | Pseudomonas aeruginosa |
| 1.13.11.1 | 50 | - |
1 h, 90% loss of activity | Pseudomonas aeruginosa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.11.1 | 1.1 | - |
3-methylcatechol | - |
Pseudomonas aeruginosa | |
| 1.13.11.1 | 5.6 | - |
4-methylcatechol | - |
Pseudomonas aeruginosa | |
| 1.13.11.1 | 7.2 | - |
catechol | - |
Pseudomonas aeruginosa | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 7 | - |
- |
Pseudomonas aeruginosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 6.5 | 10 | pH 6.5: about 25% of maximal activity, pH 10: about 70% of maximal activity | Pseudomonas aeruginosa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.13.11.1 | 6 | - |
4°C, 24 h, about 60% loss of activity | Pseudomonas aeruginosa |
| 1.13.11.1 | 7 | 9 | 4°C, 24 h, stable | Pseudomonas aeruginosa |
| 1.13.11.1 | 10 | - |
4°C, 24 h, complete loss of activity | Pseudomonas aeruginosa |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.13.11.1 | Pseudomonas aeruginosa | isoelectric point is lower than 4.0, chromatofocusing | - |
additional information |
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