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Literature summary extracted from

  • Goytia, M.; Chamond, N.; Cosson, A.; Coatnoan, N.; Hermant, D.; Berneman, A.; Minoprio, P.
    Molecular and structural discrimination of proline racemase and hydroxyproline-2-epimerase from nosocomial and bacterial pathogens (2007), PLoS ONE, 2, e885.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.4 cloned in Escherichia coli as a 6xHis-tag fusion protein Clostridioides difficile

Protein Variants

EC Number Protein Variants Comment Organism
5.1.1.8 C236S radical loss of OH-L/D-Pro epimerization Pseudomonas aeruginosa
5.1.1.8 C88S radical loss of OH-L/D-Pro epimerization Pseudomonas aeruginosa
5.1.1.8 V60F radical loss of OH-L/D-Pro epimerization. V60 residue accounts for OH-Pro ligand specificity Pseudomonas aeruginosa
5.1.1.8 V60G almost complete loss of epimerization of trans-4-hydroxy-L-Pro, some residual activity on trans-4-hydroxy-D-Pro. V60 residue accounts for OH-Pro ligand specificity Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.4 pyrrole-2-carboxylic acid
-
 Clostridioides difficile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.4 46.7
-
 (25R)-3beta-hydroxycholest-5-en-27-oate substrate: D-proline, Vmax= 0.000017 M/sec Clostridioides difficile
5.1.1.4 60.2
-
 (25R)-3beta-hydroxycholest-5-en-27-oate substrate: L-proline, Vmax = 0.000027 M/sec Clostridioides difficile
5.1.1.8 7.6
-
 trans-4-hydroxy-L-Pro 37°C Brucella suis
5.1.1.8 9
-
 trans-4-hydroxy-L-Pro 37°C Brucella melitensis
5.1.1.8 9.8
-
 trans-4-hydroxy-L-Pro 37°C Brucella abortus
5.1.1.8 14.4
-
 trans-4-hydroxy-L-Pro 37°C Burkholderia pseudomallei
5.1.1.8 14.4
-
 trans-4-hydroxy-L-Pro 37°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.4 Clostridioides difficile A8DEZ8
-
-
5.1.1.4 Clostridioides difficile VPI10463 A8DEZ8
-
-
5.1.1.8 Brucella abortus Q57B94
-
-
5.1.1.8 Brucella melitensis Q8YJ29
-
-
5.1.1.8 Brucella melitensis 16M Q8YJ29
-
-
5.1.1.8 Brucella suis Q8FYS0
-
-
5.1.1.8 Burkholderia pseudomallei Q63NG7
-
-
5.1.1.8 Pseudomonas aeruginosa Q9I476
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.4 L-proline CdPRAC from Clostridium difficile racemizes both L- and D-Pro but not OH-L/D-Pro or any other natural amino acid Clostridioides difficile D-proline
-
 r
5.1.1.4 L-proline CdPRAC from Clostridium difficile racemizes both L- and D-Pro but not OH-L/D-Pro or any other natural amino acid Clostridioides difficile VPI10463 D-proline
-
 r
5.1.1.8 trans-4-hydroxy-D-Pro
-
 Pseudomonas aeruginosa cis-4-hydroxy-L-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Brucella abortus cis-4-hydroxy-D-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Brucella melitensis cis-4-hydroxy-D-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Brucella suis cis-4-hydroxy-D-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Burkholderia pseudomallei cis-4-hydroxy-D-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Pseudomonas aeruginosa cis-4-hydroxy-D-Pro
-
 ?
5.1.1.8 trans-4-hydroxy-L-Pro
-
 Brucella melitensis 16M cis-4-hydroxy-D-Pro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.1.4 CdPRAC
-
 Clostridioides difficile
5.1.1.4 proline racemase
-
 Clostridioides difficile
5.1.1.8 HyPRE
-
 Brucella abortus
5.1.1.8 PrpA
-
 Brucella abortus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.4 37
-
 assay at Clostridioides difficile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.4 6
-
 using NaOAc Clostridioides difficile