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Literature summary extracted from

  • Pejchal, R.; Ludwig, M.L.
    Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication (2005), PLoS Biol., 3, e31.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.14 expressed in Escherichia coli BL21(DE3)Star cells Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.14 vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6) Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.14 Zn2+ requires zinc for activity Thermotoga maritima
2.1.1.14 Zn2+ requires zinc for activity Arabidopsis thaliana
2.1.1.14 Zn2+ requires zinc for activity Escherichia coli
2.1.1.14 Zn2+ requires zinc for activity Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.14 Arabidopsis thaliana O50008
-
-
2.1.1.14 Escherichia coli P25665
-
-
2.1.1.14 Saccharomyces cerevisiae P05694
-
-
2.1.1.14 Thermotoga maritima Q9X112
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.14 Zn(II)-NTA column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Thermotoga maritima tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Arabidopsis thaliana tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Escherichia coli tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?
2.1.1.14 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
 Saccharomyces cerevisiae tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.14 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
 Thermotoga maritima
2.1.1.14 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
 Arabidopsis thaliana
2.1.1.14 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
 Escherichia coli
2.1.1.14 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
-
 Saccharomyces cerevisiae
2.1.1.14 cobalamin-independent methionine synthase
-
 Thermotoga maritima
2.1.1.14 cobalamin-independent methionine synthase
-
 Arabidopsis thaliana
2.1.1.14 cobalamin-independent methionine synthase
-
 Escherichia coli
2.1.1.14 cobalamin-independent methionine synthase
-
 Saccharomyces cerevisiae
2.1.1.14 MetE
-
 Thermotoga maritima
2.1.1.14 MetE
-
 Arabidopsis thaliana
2.1.1.14 MetE
-
 Escherichia coli
2.1.1.14 MetE
-
 Saccharomyces cerevisiae
2.1.1.14 TM1286
-
 Thermotoga maritima
2.1.1.14 TM1286
-
 Arabidopsis thaliana
2.1.1.14 TM1286
-
 Escherichia coli
2.1.1.14 TM1286
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.14 additional information vitamin-B12 independent enzyme Arabidopsis thaliana
2.1.1.14 additional information vitamin-B12 independent enzyme Saccharomyces cerevisiae
2.1.1.14 additional information vitamin B12-independent enzyme Thermotoga maritima
2.1.1.14 additional information vitamin B12-independent enzyme Escherichia coli