BRENDA - Enzyme Database

Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication

Pejchal, R.; Ludwig, M.L.; PLoS Biol. 3, e31 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli BL21(DE3)Star cells
Thermotoga maritima
Crystallization (Commentary)
EC Number
Crystallization
Organism
2.1.1.14
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
Thermotoga maritima
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Zn2+
requires zinc for activity
Arabidopsis thaliana
2.1.1.14
Zn2+
requires zinc for activity
Escherichia coli
2.1.1.14
Zn2+
requires zinc for activity
Saccharomyces cerevisiae
2.1.1.14
Zn2+
requires zinc for activity
Thermotoga maritima
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.14
Arabidopsis thaliana
O50008
-
-
2.1.1.14
Escherichia coli
P25665
-
-
2.1.1.14
Saccharomyces cerevisiae
P05694
-
-
2.1.1.14
Thermotoga maritima
Q9X112
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.1.14
Zn(II)-NTA column chromatography
Thermotoga maritima
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Thermotoga maritima
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Arabidopsis thaliana
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Saccharomyces cerevisiae
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin-B12 independent enzyme
Arabidopsis thaliana
2.1.1.14
additional information
vitamin B12-independent enzyme
Escherichia coli
2.1.1.14
additional information
vitamin-B12 independent enzyme
Saccharomyces cerevisiae
2.1.1.14
additional information
vitamin B12-independent enzyme
Thermotoga maritima
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
expressed in Escherichia coli BL21(DE3)Star cells
Thermotoga maritima
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.14
additional information
vitamin-B12 independent enzyme
Arabidopsis thaliana
2.1.1.14
additional information
vitamin B12-independent enzyme
Escherichia coli
2.1.1.14
additional information
vitamin-B12 independent enzyme
Saccharomyces cerevisiae
2.1.1.14
additional information
vitamin B12-independent enzyme
Thermotoga maritima
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.1.1.14
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
Thermotoga maritima
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.1.1.14
Zn2+
requires zinc for activity
Arabidopsis thaliana
2.1.1.14
Zn2+
requires zinc for activity
Escherichia coli
2.1.1.14
Zn2+
requires zinc for activity
Saccharomyces cerevisiae
2.1.1.14
Zn2+
requires zinc for activity
Thermotoga maritima
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.14
Zn(II)-NTA column chromatography
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Thermotoga maritima
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Arabidopsis thaliana
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Escherichia coli
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?
2.1.1.14
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
-
676741
Saccharomyces cerevisiae
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
-
-
-
?