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Literature summary extracted from

  • Zhang, L.; Yang, B.; Lu, B.; Kai, G.; Wang, Z.; Xia, Y.; Ding, R.; Zhang, H.; Sun, X.; Chen, W.; Tang, K.
    Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent (2006), Planta, 225, 887-896.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.53 methyl jasmonate considerable increase of PMTase either in the transgenic hairy roots or the control Hyoscyamus niger
2.1.1.53 methyl jasmonate considerably increases PMTase in tobacco pmt overexpressed Hyoscyamus niger plants Nicotiana tabacum
2.1.1.53 methyl jasmonate enhances PMTase activity Nicotiana tabacum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.53 tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain Nicotiana tabacum
2.1.1.53 tobacco pmt gene with a selectable marker (nptII) gene and the T-DNA of pRiA4 of Agrobacterium rhizogenes introduced into Hyoscyamus niger leaf explants using disarmed Agrobacterium tumefaciens C58C1 strain Hyoscyamus niger

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.53 additional information cDNA from Nicotiana tabacum encoding PMT is introduced into the genome of a scopolamine-producing Hyoscyamus niger mediated by the disarmed Agrobacterium tumefaciens strain C58C1, which also carries Agrobacterium rhizogenes Ri plasmid pRiA4, and expressed under the control of the CaMV 35S promoter. Hairy root lines transformed with pmt present 5fold higher PMT activity than the control, and the methylputrescine levels of the resulting engineered hairy roots increase up to 5fold compared to the control and wild-type roots, but no increase in tropane alkaloids measured. After methyl jasmonate treatment, a considerable increase of PMTase and endogenous H6Hase as well as an increase in scopolamine content is found either in the transgenic hairy roots or the control Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.53 Hyoscyamus niger
-
-
-
2.1.1.53 Nicotiana tabacum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.53 root
-
 Nicotiana tabacum
-
2.1.1.53 root PMT activity in transgenic hairy root cultures is 5fold higher than that in the wild-type root Hyoscyamus niger
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Hyoscyamus niger S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.53 PMT
-
 Nicotiana tabacum
2.1.1.53 PMT
-
 Hyoscyamus niger
2.1.1.53 putrescine N-methyltransferase
-
 Nicotiana tabacum