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Literature summary extracted from

  • Dorion, S.; Matton, D.P.; Rivoal, J.
    Characterization of a cytosolic nucleoside diphosphate kinase associated with cell division and growth in potato (2006), Planta, 224, 108-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expression in Escherichia coli as His-tagged protein Solanum chacoense

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 UDP
-
Solanum chacoense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 0.075
-
GTP
-
Solanum chacoense
2.7.4.6 0.21
-
UDP
-
Solanum chacoense
2.7.4.6 0.4
-
ATP
-
Solanum chacoense
2.7.4.6 1.03
-
CTP
-
Solanum chacoense
2.7.4.6 1.17
-
TDP
-
Solanum chacoense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 cytosol
-
Solanum chacoense 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 19300
-
x * 19300, SDS-PAGE of recombinant protein including His-tag Solanum chacoense

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Solanum chacoense Q3Y5A4 isoform NDPK1, strongly regulated by the NTP/NDP ratio
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant protein Solanum chacoense

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 cell culture enzyme activity and protein level raise during exponential growth, no rise in activity when supply with sucrose is limited Solanum chacoense
-
2.7.4.6 leaf
-
Solanum chacoense
-
2.7.4.6 root predominant localization in meristematic zones and provascular tissues Solanum chacoense
-
2.7.4.6 tuber
-
Solanum chacoense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ADP + CTP
-
Solanum chacoense ATP + CDP
-
?
2.7.4.6 ADP + GTP
-
Solanum chacoense ATP + GDP
-
?
2.7.4.6 ATP + nucleoside diphosphate
-
Solanum chacoense ADP + nucleoside triphosphate
-
?
2.7.4.6 ATP + TDP
-
Solanum chacoense ADP + TTP
-
?
2.7.4.6 ATP + UDP
-
Solanum chacoense ADP + UTP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 ? x * 19300, SDS-PAGE of recombinant protein including His-tag Solanum chacoense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 6.5 8 broad Solanum chacoense