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Literature summary extracted from

  • Stenzel, O.; Teuber, M.; Draeger, B.
    Putrescine N-methyltransferase in Solanum tuberosum L., a calystegine-forming plant (2006), Planta, 223, 200-212.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.53 additional information chimera proteins obtained by fusion of fragments of Solanum tuberosum pmt2 and Hyoscyamus niger pmt are active as PMT Hyoscyamus niger
2.1.1.53 additional information no functional protein translated from Solanum tuberosum pmt2, chimera proteins obtained by fusion of fragments of Solanum tuberosum pmt2 and Hyoscyamus niger pmt are active as PMT, if the initial part of pmt2 is used, indicating that a mutation in the terminal part of the gene causes insolubility of the enzyme Solanum tuberosum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.53
-
 Hyoscyamus niger
2.1.1.53 pmt1 and pmt2 cloned into pET-21d vector, PMT1 expressed in Escherichia coli Rosetta-gami (DE3), PMT2 expressed in Escherichia coli BL21 (DE3) and Rosetta-gami (DE3), pmt1 yields active enzymes, while pmt2 expression results in insoluble protein Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.53 1,6-diaminohexane weaker inhibitor Solanum tuberosum
2.1.1.53 1,7-Diaminoheptane weaker inhibitor Solanum tuberosum
2.1.1.53 cadaverine strong inhibitor Solanum tuberosum
2.1.1.53 n-butylamine strong inhibitor Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.53 0.25
-
 putrescine PMT1 Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.53 38180
-
-
 Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.53 Hyoscyamus niger Q9XJ41
-
-
2.1.1.53 Solanum tuberosum Q70AR0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.53
-
 Hyoscyamus niger
2.1.1.53 by Ni2+ chelate affinity chromatography Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.53 root very weak signal Solanum tuberosum
-
2.1.1.53 tuber transcript only in potato tuber eyes at sprouting start and in 1 and 3 mm long sprouts Solanum tuberosum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.53 additional information inactive with n-butylamine, 1,6-diaminohexane and 1,7-diaminoheptane, the PMT enzyme of potato appears to be specific for putrescine, it does not accept other mono-, di- or tri-amines Solanum tuberosum ?
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Hyoscyamus niger S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?
2.1.1.53 S-adenosyl-L-methionine + putrescine
-
 Solanum tuberosum S-adenosyl-L-homocysteine + N-methylputrescine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.53 PMT
-
 Hyoscyamus niger
2.1.1.53 PMT1
-
 Solanum tuberosum
2.1.1.53 PMT2
-
 Solanum tuberosum