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Literature summary extracted from

  • Greenshields, D.L.; Liu, G.; Selvaraj, G.; Wei, Y.
    Differential regulation of wheat quinone reductases in response to powdery mildew infection (2005), Planta, 222, 867-875.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 expression in Escherichia coli Triticum monococcum
1.6.5.5 expression in Escherichia coli, expression of TmQR1 is lethal to the cells, it is not possible to purify enough protein for further analysis Triticum monococcum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 dicoumarol
-
 Triticum monococcum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.00336
-
 menadione pH 7.5, 25°C Triticum monococcum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.2 additional information Triticum monococcum TmQR2 could play a role in protecting the infected epidermis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Triticum monococcum Q5EI63
-
-
1.6.5.5 Triticum monococcum Q56D13
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 epidermis the gene for QR2 is isolated from an expressed sequence tag collection derived from the epidermis of a diploid Triticum monococcum L. 24 h after inoculation with the powdery mildew fungus Blumeria graminis EO Speer f. sp. tritici Em. Marchal. TmQR1 is repressed while TmQR2 is induced in the epidermis during powdery mildew infection. TmQR2 can play a role in protecting the infected epidermis Triticum monococcum
-
1.6.5.5 epidermis the gene for QR1 is isolated from an expressed sequence tag collection derived from the epidermis of a diploid Triticum monococcum L. 24 h after inoculation with the powdery mildew fungus Blumeria graminis EO Speer f. sp. tritici Em. Marchal. TmQR1 is repressed while TmQR2 is induced in the epidermis during powdery mildew infection Triticum monococcum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 menadione + NADH + H+
-
 Triticum monococcum ? + NAD+
-
 ?
1.6.5.2 menadione + NADPH + H+
-
 Triticum monococcum ? + NADP+
-
 ?
1.6.5.2 additional information TmQR2 could play a role in protecting the infected epidermis Triticum monococcum ?
-
 ?
1.6.5.5 additional information QR1 catalyzes the univalent reduction of quinones to semiquinone radicals Triticum monococcum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 TmQR2
-
 Triticum monococcum
1.6.5.5 TmQR1
-
 Triticum monococcum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.5.2 25
-
 assay at Triticum monococcum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 7.5
-
 assay at Triticum monococcum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 NADH can use either NADH or NADPH as an electron donor Triticum monococcum
1.6.5.2 NADPH can use either NADH or NADPH as an electron donor Triticum monococcum