BRENDA - Enzyme Database

Cytosolic pyruvate kinase: subunit composition, activity, and amount in developing castor and soybean seeds, and biochemical characterization of the purified castor seed enzyme

Turner, W.L.; Knowles, V.L.; Plaxton, W.C.; Planta 222, 1051-1062 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
aspartate
allosteric activator
Ricinus communis
General Stability
EC Number
General Stability
Organism
2.7.1.40
25 mM MES and 25 mM Bis-Tris propane as the assay buffer
Ricinus communis
2.7.1.40
at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of
Ricinus communis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
80% activity at pH 6.4, 86% activity at pH 7.4
Ricinus communis
2.7.1.40
ATP
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4
Ricinus communis
2.7.1.40
citrate
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4
Ricinus communis
2.7.1.40
D-fructose 1,6-bisphosphate
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4
Ricinus communis
2.7.1.40
diphosphate
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4
Ricinus communis
2.7.1.40
glutamate
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4
Ricinus communis
2.7.1.40
isocitrate
76% activity at pH 6.4, 91% activity at pH 7.4
Ricinus communis
2.7.1.40
malate
78% activity at pH 6.4, 90% activity at pH 7.4
Ricinus communis
2.7.1.40
additional information
not inhibited by 5 mM ADP
Ricinus communis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.046
-
ADP
at pH 7.4
Ricinus communis
2.7.1.40
0.046
-
phosphoenolpyruvate
at pH 7.4
Ricinus communis
2.7.1.40
0.055
-
ADP
at pH 7.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.082
-
phosphoenolpyruvate
at pH 6.4
Ricinus communis
2.7.1.40
0.09
-
ADP
at pH 6.4
Ricinus communis
2.7.1.40
0.093
-
phosphoenolpyruvate
at pH 7.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.101
-
ADP
at pH 6.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.15
-
phosphoenolpyruvate
at pH 6.4, in the presence of 10 mM glutamate
Ricinus communis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
cytosol
-
Glycine max
5829
-
2.7.1.40
cytosol
-
Ricinus communis
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
additional information
activity is independent of free Mg2+ concentration in the range 4-20 mM
Glycine max
2.7.1.40
additional information
activity is independent of free Mg2+ concentration in the range 4-20 mM
Ricinus communis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
56000
-
4 * 56000, gel filtration
Glycine max
2.7.1.40
56000
-
4 * 56000, gel filtration
Ricinus communis
2.7.1.40
230000
-
gel filtration
Glycine max
2.7.1.40
230000
-
gel filtration
Ricinus communis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.40
Glycine max
-
-
-
2.7.1.40
Ricinus communis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.40
Butyl-Sepharose chromatography and Fractogel EMD DEAE-650 (S) anion-exchange chromatography
Ricinus communis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cotyledon
-
Ricinus communis
-
2.7.1.40
embryo
-
Glycine max
-
2.7.1.40
endosperm
developing endosperm
Ricinus communis
-
2.7.1.40
seed
-
Glycine max
-
2.7.1.40
seed
-
Ricinus communis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.40
0.039
-
clarified extract
Ricinus communis
2.7.1.40
73.1
-
after 1874fold purification
Ricinus communis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
676689
Glycine max
ATP + pyruvate
-
-
-
?
2.7.1.40
ADP + phosphoenolpyruvate
-
676689
Ricinus communis
ATP + pyruvate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.40
homotetramer
4 * 56000, gel filtration
Glycine max
2.7.1.40
homotetramer
4 * 56000, gel filtration
Ricinus communis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.4
7.4
displays a broad pH optimum of 6.4, however, more efficient substrate utilization is observed at pH 7.4
Ricinus communis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
5
8.25
-
Ricinus communis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ADP
-
Ricinus communis
2.7.1.40
ADP
-
Glycine max
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
1.2
-
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4
Ricinus communis
glutamate
2.7.1.40
8
-
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4
Ricinus communis
D-fructose 1,6-bisphosphate
2.7.1.40
13.7
-
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4
Ricinus communis
ATP
2.7.1.40
14.2
-
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4
Ricinus communis
citrate
2.7.1.40
17.2
-
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4
Ricinus communis
diphosphate
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.40
aspartate
allosteric activator
Ricinus communis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.40
ADP
-
Ricinus communis
2.7.1.40
ADP
-
Glycine max
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.40
25 mM MES and 25 mM Bis-Tris propane as the assay buffer
Ricinus communis
2.7.1.40
at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of
Ricinus communis
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
2.7.1.40
1.2
-
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4
Ricinus communis
glutamate
2.7.1.40
8
-
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4
Ricinus communis
D-fructose 1,6-bisphosphate
2.7.1.40
13.7
-
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4
Ricinus communis
ATP
2.7.1.40
14.2
-
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4
Ricinus communis
citrate
2.7.1.40
17.2
-
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4
Ricinus communis
diphosphate
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.40
3-phosphoglycerate
80% activity at pH 6.4, 86% activity at pH 7.4
Ricinus communis
2.7.1.40
ATP
IC50: 8.8 mM at pH 6.4, IC50: 13.7 mM at pH 7.4
Ricinus communis
2.7.1.40
citrate
IC50: 9.2 mM at pH 6.4, IC50: 14.2 mM at pH 7.4
Ricinus communis
2.7.1.40
D-fructose 1,6-bisphosphate
IC50: 8.4 mM at pH 6.4, IC50: 8.0 mM at pH 7.4
Ricinus communis
2.7.1.40
diphosphate
IC50: 9.8 mM at pH 6.4, IC50: 17.2 mM at pH 7.4
Ricinus communis
2.7.1.40
glutamate
IC50: 2.5 mM at pH 6.4, IC50: 1.2 mM at pH 7.4
Ricinus communis
2.7.1.40
isocitrate
76% activity at pH 6.4, 91% activity at pH 7.4
Ricinus communis
2.7.1.40
malate
78% activity at pH 6.4, 90% activity at pH 7.4
Ricinus communis
2.7.1.40
additional information
not inhibited by 5 mM ADP
Ricinus communis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.40
0.046
-
ADP
at pH 7.4
Ricinus communis
2.7.1.40
0.046
-
phosphoenolpyruvate
at pH 7.4
Ricinus communis
2.7.1.40
0.055
-
ADP
at pH 7.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.082
-
phosphoenolpyruvate
at pH 6.4
Ricinus communis
2.7.1.40
0.09
-
ADP
at pH 6.4
Ricinus communis
2.7.1.40
0.093
-
phosphoenolpyruvate
at pH 7.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.101
-
ADP
at pH 6.4, in the presence of 10 mM glutamate
Ricinus communis
2.7.1.40
0.15
-
phosphoenolpyruvate
at pH 6.4, in the presence of 10 mM glutamate
Ricinus communis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.40
cytosol
-
Glycine max
5829
-
2.7.1.40
cytosol
-
Ricinus communis
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.40
additional information
activity is independent of free Mg2+ concentration in the range 4-20 mM
Glycine max
2.7.1.40
additional information
activity is independent of free Mg2+ concentration in the range 4-20 mM
Ricinus communis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.40
56000
-
4 * 56000, gel filtration
Glycine max
2.7.1.40
56000
-
4 * 56000, gel filtration
Ricinus communis
2.7.1.40
230000
-
gel filtration
Glycine max
2.7.1.40
230000
-
gel filtration
Ricinus communis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.40
Butyl-Sepharose chromatography and Fractogel EMD DEAE-650 (S) anion-exchange chromatography
Ricinus communis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.40
cotyledon
-
Ricinus communis
-
2.7.1.40
embryo
-
Glycine max
-
2.7.1.40
endosperm
developing endosperm
Ricinus communis
-
2.7.1.40
seed
-
Glycine max
-
2.7.1.40
seed
-
Ricinus communis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.40
0.039
-
clarified extract
Ricinus communis
2.7.1.40
73.1
-
after 1874fold purification
Ricinus communis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.40
ADP + phosphoenolpyruvate
-
676689
Glycine max
ATP + pyruvate
-
-
-
?
2.7.1.40
ADP + phosphoenolpyruvate
-
676689
Ricinus communis
ATP + pyruvate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.40
homotetramer
4 * 56000, gel filtration
Glycine max
2.7.1.40
homotetramer
4 * 56000, gel filtration
Ricinus communis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.40
6.4
7.4
displays a broad pH optimum of 6.4, however, more efficient substrate utilization is observed at pH 7.4
Ricinus communis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.40
5
8.25
-
Ricinus communis