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Literature summary extracted from

  • Provan, F.; Haavik, J.; Lillo, C.
    The regulatory phosphorylated serine in full-length nitrate reductase is necessary for optimal binding to a 14-3-3 protein (2006), Plant Sci., 170, 394-398.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.1 expressed in Nicotiana plumbaginifolia Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.1 S521D the protein is constitutively active under conditions where the wild type protein would be rapidly inactivated, such as in the dark Nicotiana tabacum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 14-3-3 protein BMH1 0.0018 mM, reduces activity to less than 20%, at pH 6.0 Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.1 Mg2+ 1 mM Nicotiana tabacum

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1 ammonium sulfate precipitation and Ble Sepharose chromatography Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Nicotiana tabacum nitrite + NAD+ + H2O
-
 ?
1.7.1.1 nitrite + NADH
-
 Nicotiana tabacum nitric oxide + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Nicotiana tabacum