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Literature summary extracted from

  • Obana, Y.; Omoto, D.; Kato, C.; Matsumoto, K.; Nagai, Y.; Kavakli, I.H.; Hamada, S.; Edwards, G.E.; Okita, T.W.; Matsui, H.; Ito, H.
    Enhanced turnover of transitory starch by expression of up-regulated ADP-glucose pyrophosphorylases in Arabidopsis thaliana (2006), Plant Sci., 170, 1-11.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate wild-type, half-maximal activation at 0.016 mM, mutant A33K, at 0.0055 mM, mutant G96N, at 0.0064 mM, mutant A33K/G96N, at 0.0066 mM Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 A33K mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates Arabidopsis thaliana
2.7.7.27 A33K/G96N mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates. Double mutant is less susceptible for inhibition by phosphate and shows increased sensitivity to 3-phosphoglycerate Arabidopsis thaliana
2.7.7.27 G96N mutation in leaf-specific large subunit ApL1, little effect on affinity of holoenzyme for substrates Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 phosphate wild-type, 50% inhibition at 0.044 mM, mutant A33K, 50% inhibition at 0.043 mM, mutant G96N, 50% inhibition at 0.054 mM, mutant A33K/G96N, 50% inhibition at 0.268 mM, all in presence of 3-phosphoglycerate in at concentration of halfmaximal activation Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.041
-
 alpha-D-glucose-1-phosphate mutant A33K, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.052
-
 ATP mutant G96N, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.055
-
 ATP mutant A33K/G96N, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.055
-
 alpha-D-glucose-1-phosphate mutant G96N, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.056
-
 ATP mutant A33K, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.063
-
 alpha-D-glucose-1-phosphate mutant A33K/G96N, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.073
-
 ATP wild-type, pH 7.5 Arabidopsis thaliana
2.7.7.27 0.083
-
 alpha-D-glucose-1-phosphate wild-type, pH 7.5 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Arabidopsis thaliana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose-1-phosphate
-
 Arabidopsis thaliana diphosphate + ADP-glucose
-
 ?