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Literature summary extracted from

  • Engprasert, S.; Taura, F.; Shoyama, Y.
    Molecular cloning, expression and characterization of recombinant 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Coleus forskohlii Briq. (2005), Plant Sci., 169, 287-294.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space Plectranthus barbatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.267 EDTA
-
Plectranthus barbatus
1.1.1.267 fosmidomycin IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo Plectranthus barbatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.267 0.0825
-
NADPH pH 8.0, 37°C, recombinant enzyme Plectranthus barbatus
1.1.1.267 0.147
-
1-deoxy-D-xylulose 5-phosphate pH 8.0, 37°C, recombinant enzyme Plectranthus barbatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 chloroplast
-
Plectranthus barbatus 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.267 Ca2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
1.1.1.267 Co2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
1.1.1.267 Mg2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus
1.1.1.267 Mn2+ activates, a divalent cation is required for activity, best cation, can partially be substituted by other divalent cations Plectranthus barbatus
1.1.1.267 additional information no activity with Fe2+ and Cu2+ Plectranthus barbatus
1.1.1.267 Zn2+ activates, a divalent cation is required for activity, can partially substitute for Mn2+ Plectranthus barbatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.267 54000
-
x * 54000, recombinant enzyme, SDS-PAGE Plectranthus barbatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Plectranthus barbatus the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Plectranthus barbatus Q6R3F4 i.e. Plectranthus barbatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.267 recombinant C-terminally His-tagged enzyme 8.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultracentrifugation and dialysis, followed by anion exchange and hydroxylapatite chromatography, affinity chromatography, and again dialysis Plectranthus barbatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.267 leaf high expression level Plectranthus barbatus
-
1.1.1.267 additional information quantitative expression analysis Plectranthus barbatus
-
1.1.1.267 root very low expression level Plectranthus barbatus
-
1.1.1.267 stem moderate expression level Plectranthus barbatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.267 additional information
-
quantitative analysis of forskolin production in plant tissues Plectranthus barbatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ NADPH is the preferred cofactor Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+
-
?
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview Plectranthus barbatus 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.267 ? x * 54000, recombinant enzyme, SDS-PAGE Plectranthus barbatus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 DXR
-
Plectranthus barbatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.267 37 45 recombinant enzyme Plectranthus barbatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.267 7.8 8.3 recombinant enzyme Plectranthus barbatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADH 15% of the activity with NADPH Plectranthus barbatus
1.1.1.267 NADPH preferred cofactor Plectranthus barbatus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.267 0.00045
-
IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo Plectranthus barbatus fosmidomycin