Literature summary extracted from
Engprasert, S.; Taura, F.; Shoyama, Y.
Molecular cloning, expression and characterization of recombinant 1-deoxy-D-xylulose-5-phosphate reductoisomerase from Coleus forskohlii Briq. (2005), Plant Sci., 169, 287-294.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.267 |
DNA and amino acid sequence determination and analysis, quantitative expression analysis, expression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) periplasmic space |
Plectranthus barbatus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.1.1.267 |
EDTA |
- |
Plectranthus barbatus |
|
1.1.1.267 |
fosmidomycin |
IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo |
Plectranthus barbatus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.1.1.267 |
0.0825 |
- |
NADPH |
pH 8.0, 37°C, recombinant enzyme |
Plectranthus barbatus |
|
1.1.1.267 |
0.147 |
- |
1-deoxy-D-xylulose 5-phosphate |
pH 8.0, 37°C, recombinant enzyme |
Plectranthus barbatus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.1.1.267 |
chloroplast |
- |
Plectranthus barbatus |
9507 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.1.1.267 |
Ca2+ |
activates, a divalent cation is required for activity, can partially substitute for Mn2+ |
Plectranthus barbatus |
|
1.1.1.267 |
Co2+ |
activates, a divalent cation is required for activity, can partially substitute for Mn2+ |
Plectranthus barbatus |
|
1.1.1.267 |
Mg2+ |
activates, a divalent cation is required for activity, can partially substitute for Mn2+ |
Plectranthus barbatus |
|
1.1.1.267 |
Mn2+ |
activates, a divalent cation is required for activity, best cation, can partially be substituted by other divalent cations |
Plectranthus barbatus |
|
1.1.1.267 |
additional information |
no activity with Fe2+ and Cu2+ |
Plectranthus barbatus |
|
1.1.1.267 |
Zn2+ |
activates, a divalent cation is required for activity, can partially substitute for Mn2+ |
Plectranthus barbatus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.1.1.267 |
54000 |
- |
x * 54000, recombinant enzyme, SDS-PAGE |
Plectranthus barbatus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.1.1.267 |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
Plectranthus barbatus |
the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.267 |
Plectranthus barbatus |
Q6R3F4 |
i.e. Plectranthus barbatus |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.267 |
recombinant C-terminally His-tagged enzyme 8.7fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultracentrifugation and dialysis, followed by anion exchange and hydroxylapatite chromatography, affinity chromatography, and again dialysis |
Plectranthus barbatus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.1.1.267 |
leaf |
high expression level |
Plectranthus barbatus |
- |
1.1.1.267 |
additional information |
quantitative expression analysis |
Plectranthus barbatus |
- |
1.1.1.267 |
root |
very low expression level |
Plectranthus barbatus |
- |
1.1.1.267 |
stem |
moderate expression level |
Plectranthus barbatus |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.1.1.267 |
additional information |
- |
quantitative analysis of forskolin production in plant tissues |
Plectranthus barbatus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.267 |
1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ |
NADPH is the preferred cofactor |
Plectranthus barbatus |
2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ |
- |
? |
|
1.1.1.267 |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ |
the enzyme catalyzes the first committed step in the biosynthesis of isopentenyl diphosphate in the plastidial 2C-methyl-D-erythritol 4-phosphate pathway leading to production of forskolin, overview |
Plectranthus barbatus |
2-C-methyl-D-erythritol 4-phosphate + NADP+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.267 |
? |
x * 54000, recombinant enzyme, SDS-PAGE |
Plectranthus barbatus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.267 |
DXR |
- |
Plectranthus barbatus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.1.1.267 |
37 |
45 |
recombinant enzyme |
Plectranthus barbatus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.1.1.267 |
7.8 |
8.3 |
recombinant enzyme |
Plectranthus barbatus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.267 |
NADH |
15% of the activity with NADPH |
Plectranthus barbatus |
|
1.1.1.267 |
NADPH |
preferred cofactor |
Plectranthus barbatus |
|
IC50 Value
EC Number |
IC50 Value |
IC50 Value Maximum |
Comment |
Organism |
Inhibitor |
Structure |
---|
1.1.1.267 |
0.00045 |
- |
IC50: 450 nM, recombinant enzyme, inhibition of growth and forskolin production in vivo |
Plectranthus barbatus |
fosmidomycin |
|