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Literature summary extracted from

  • Kim, B.G.; Lee, H.J.; Park, Y.; Lim, Y.; Ahn, J.H.
    Characterization of an O-methyltransferase from soybean (2006), Plant Physiol. Biochem., 44, 236-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.42 SOMT-9, DNA and amino acid sequence determination, expression of GST-tagged SOMT-9 in Escherichia coli Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.42 64000
-
 x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.42 quercetin + S-adenosyl-L-methionine Glycine max SOMT-9 is involved in the biosynthesis of isorhamnetin 3'-O-methylquercetin + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.42 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.42 recombinant GST-tagged SOMT-9 from Escherichia coli to homogeneity Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.42 leaf
-
 Glycine max
-
2.1.1.42 root
-
 Glycine max
-
2.1.1.42 stem
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.42 luteolin + S-adenosyl-L-methionine high 3'-O-methyltransferase activity Glycine max 3'-O-methyl luteolin + S-adenosyl-L-homocysteine i.e. chrysoeriol ?
2.1.1.42 additional information SOMT-9 transfers a methyl group to the 3'-OH group of substrates having ortho-hydroxyl groups and uses a flavonoids as a substrates, no activity with 3,3'-dihydroxyflavone, 5,3'-dihydroxyflavone, 6,3'-dihydroxyflavone, 3'-hydroxyflavone, and 3'-hydroxyflavanone Glycine max ?
-
 ?
2.1.1.42 quercetin + S-adenosyl-L-methionine SOMT-9 is involved in the biosynthesis of isorhamnetin Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine
-
 ?
2.1.1.42 quercetin + S-adenosyl-L-methionine a flavone, 3'-O-methyltransferase activity, best substrate Glycine max 3'-O-methylquercetin + S-adenosyl-L-homocysteine i.e. isorhamnetin ?
2.1.1.42 taxifolin + S-adenosyl-L-methionine 3'-O-methyltransferase activity Glycine max 3'-O-methyl taxifolin + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.42 ? x * 64000, recombinant GST-tagged SOMT-9, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.1.1.42 3'-O-methyltransferase
-
 Glycine max
2.1.1.42 SOMT-9
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.42 37
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.42 7.8
-
 assay at Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.42 S-adenosyl-L-methionine
-
 Glycine max