Literature summary extracted from
Schofield, A.; Paliyath, G.
Modulation of carotenoid biosynthesis during tomato fruit ripening through phytochrome regulation of phytoene synthase activity (2005), Plant Physiol. Biochem., 43, 1052-1060.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
ATP |
stimulatory to PSY activity above 5 mM |
Solanum lycopersicum |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
ATP |
20 mM, inhibitory effect |
Solanum lycopersicum |
|
2.5.1.32 |
Mg2+ |
50 mM, inhibitory effect |
Solanum lycopersicum |
|
2.5.1.32 |
Triton X-100 |
0.2%, total inhibition |
Solanum lycopersicum |
|
2.5.1.32 |
Tween 20 |
0.2%, total inhibition |
Solanum lycopersicum |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
0.0374 |
- |
geranylgeranyl diphosphate |
in 400 mM Tris (pH 6.5), 10 mM sodium ascorbate, 5 mM MgCl2, 0.5 mM MnCl2, 3 mM ATP and 0.02 mM FAD |
Solanum lycopersicum |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.5.1.32 |
Mg2+ |
1 mM, 40-50% stimulating effect |
Solanum lycopersicum |
|
2.5.1.32 |
additional information |
Mn2+ does not have any stimulatory effect |
Solanum lycopersicum |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.32 |
Solanum lycopersicum |
P08196 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.32 |
Sephadex G-25 gel filtration and ammonium sulfate fractionation |
Solanum lycopersicum |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.5.1.32 |
fruit |
- |
Solanum lycopersicum |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.5.1.32 |
geranylgeranyl diphosphate + geranylgeranyl diphosphate |
- |
Solanum lycopersicum |
prephytoene diphosphate + diphosphate |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.32 |
PSY |
- |
Solanum lycopersicum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.5.1.32 |
6.5 |
8.5 |
- |
Solanum lycopersicum |