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Literature summary extracted from

  • de Kraker, J.W.; Luck, K.; Textor, S.; Tokuhisa, J.G.; Gershenzon, J.
    Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine (2007), Plant Physiol., 143, 970-986.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.13 additional information knockout-mutant of isoform IPMS1 shows an increase in valine content, but no change in leucine content. Insertion mutation for isoform IPMS2 do not show any changes in soluble amino acid content. Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 EDTA 10 mM, complete loss of activity; 10 mM, complete loss of activity Arabidopsis thaliana
2.3.3.13 Leu inhibition of both enzymes reaches a maximum of 30% to 35% around 1 mM Leu; inhibition of both enzymes reaches a maximum of 30% to 35% around 1 mM Leu Arabidopsis thaliana
2.3.3.13 leucine 1 mM, 30-35% inhibition, both isoforms IPMS1 and IPMS2 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.13 0.016
-
acetyl-CoA
-
Arabidopsis thaliana
2.3.3.13 0.016
-
acetyl-CoA pH 8.0, isoform IPMS2 Arabidopsis thaliana
2.3.3.13 0.045
-
acetyl-CoA
-
Arabidopsis thaliana
2.3.3.13 0.045
-
acetyl-CoA pH 8.0, isoform IPMS1 Arabidopsis thaliana
2.3.3.13 0.279
-
2-oxo-3-methylbutanoate
-
Arabidopsis thaliana
2.3.3.13 0.279
-
2-oxo-3-methylbutanoate pH 8.0, isoform IPMS2 Arabidopsis thaliana
2.3.3.13 0.304
-
2-oxo-3-methylbutanoate
-
Arabidopsis thaliana
2.3.3.13 0.304
-
2-oxo-3-methylbutanoate pH 8.0, isoform IPMS1 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.13 chloroplast
-
Arabidopsis thaliana 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 K+ 4 mM, slight stimulation Arabidopsis thaliana
2.3.3.13 Mg2+ required, both isoforms IPMS1 and IPMS2 Arabidopsis thaliana
2.3.3.13 Mg2+ requires Mg2+ in millimolar concentrations Arabidopsis thaliana
2.3.3.13 Mn2+ after treatment with EDTA, 4 mM Mn2+ restores about 50% of the initial activity Arabidopsis thaliana
2.3.3.13 Mn2+ after tretment with EDTA, 4 mM Mn2+ restores about 50% of the initial activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.13 62300
-
2 * 62300, calculated from nucleotide sequence Arabidopsis thaliana
2.3.3.13 63100
-
2 * 63100, calculated, isoform IPMS1 with His-tag Arabidopsis thaliana
2.3.3.13 64100
-
4 * 64100, calculated, isoform IPMS2 with His-tag Arabidopsis thaliana
2.3.3.13 64100
-
4 * 64100, calculated from nucleotide sequence Arabidopsis thaliana
2.3.3.13 124000
-
gel filtration Arabidopsis thaliana
2.3.3.13 124000
-
gel filtration, isoform IPMS1 Arabidopsis thaliana
2.3.3.13 280000
-
gel filtration Arabidopsis thaliana
2.3.3.13 280000
-
gel filtration, isoform IPMS2 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Arabidopsis thaliana
-
isoform IPMS1 and isoform IPMS2
-
2.3.3.13 Arabidopsis thaliana Q9C550
-
-
2.3.3.13 Arabidopsis thaliana Q9LPR4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13
-
Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Arabidopsis thaliana 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2.3.3.13 2-oxo-butanoate + acetyl-CoA + H2O isoform IPMS1, 112% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 97.5% of the rate with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-ethyl-2-hydroxysuccinic acid + CoA
-
?
2.3.3.13 2-oxo-butanoate + acetyl-CoA + H2O 112.2% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-ethyl-2-hydroxysuccinic acid + CoA
-
?
2.3.3.13 2-oxo-butanoate + acetyl-CoA + H2O 97.5% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-ethyl-2-hydroxysuccinic acid + CoA
-
?
2.3.3.13 2-oxo-n-pentanoate + acetyl-CoA + H2O isoform IPMS1, 27.2% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 33.8% of the rate with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-hydroxy-2-propylsuccinic acid + CoA
-
?
2.3.3.13 2-oxovalerate + acetyl-CoA + H2O 27.2% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana ?
-
?
2.3.3.13 2-oxovalerate + acetyl-CoA + H2O 33.8% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana ?
-
?
2.3.3.13 4-methylthio-2-oxopentanoate + acetyl-CoA + H2O
-
Arabidopsis thaliana ? + CoA at very low rate, reaction is involved in glucosinolate chain elongation ?
2.3.3.13 pyruvate + acetyl-CoA + H2O isoform IPMS1, 12.7% of the rate with 2-oxo-3-methylbutanoate, isoform IPMS2, 15.7% of the rate with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-hydroxy-2-methylsuccinic acid + CoA
-
?
2.3.3.13 pyruvate + acetyl-CoA + H2O 12.7% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-hydroxy-2-methylsuccinic acid + CoA
-
?
2.3.3.13 pyruvate + acetyl-CoA + H2O 15.7% of the activity with 2-oxo-3-methylbutanoate Arabidopsis thaliana 2-hydroxy-2-methylsuccinic acid + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.13 dimer 2 * 63100, calculated, isoform IPMS1 with His-tag Arabidopsis thaliana
2.3.3.13 dimer 2 * 62300, calculated from nucleotide sequence Arabidopsis thaliana
2.3.3.13 tetramer 4 * 64100, calculated, isoform IPMS2 with His-tag Arabidopsis thaliana
2.3.3.13 tetramer 4 * 64100, calculated from nucleotide sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 2-isopropylmalate synthase 1, chloroplastic
-
Arabidopsis thaliana
2.3.3.13 2-isopropylmalate synthase 2, chloroplastic
-
Arabidopsis thaliana
2.3.3.13 IPMS1
-
Arabidopsis thaliana
2.3.3.13 IPMS2
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 1.9
-
acetyl-CoA
-
Arabidopsis thaliana
2.3.3.13 1.9
-
acetyl-CoA pH 8.0, isoform IPMS2 Arabidopsis thaliana
2.3.3.13 2.2
-
acetyl-CoA
-
Arabidopsis thaliana
2.3.3.13 2.2
-
acetyl-CoA pH 8.0, isoform IPMS1 Arabidopsis thaliana
2.3.3.13 2.3
-
2-oxo-3-methylbutanoate
-
Arabidopsis thaliana
2.3.3.13 2.3
-
2-oxo-3-methylbutanoate pH 8.0, isoform IPMS2 Arabidopsis thaliana
2.3.3.13 2.4
-
2-oxo-3-methylbutanoate
-
Arabidopsis thaliana
2.3.3.13 2.4
-
2-oxo-3-methylbutanoate pH 8.0, isoform IPMS1 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.13 8.5
-
-
Arabidopsis thaliana
2.3.3.13 8.5
-
both isoforms IPMS1 and IPMS2 Arabidopsis thaliana