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Literature summary extracted from

  • Eisenhut, M.; Kahlon, S.; Hasse, D.; Ewald, R.; Lieman-Hurwitz, J.; Ogawa, T.; Ruth, W.; Bauwe, H.; Kaplan, A.; Hagemann, M.
    The plant-like C2 glycolate cycle and the bacterial-like glycerate pathway cooperate in phosphoglycolate metabolism in cyanobacteria (2006), Plant Physiol., 142, 333-342.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.4.4.2 additional information GDC is dispensable for cyanobacterial metabolism Synechocystis sp.
2.1.2.1 degradation essential for one-carbon metabolism Synechocystis sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.14 gene glcD, expression of N-terminally Strep-tagged enzyme in Escherichia coli strain BL21 Synechocystis sp.
2.1.2.1 expressed as fusion protein with an N-terminal Strep-tag in Escherichia coli strain BL21 Gold Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.99.14 additional information construction of GlcD-deficient mutants, co-mutation of other enzymes of the alternative bacterial glycerate pathway, progressive segregation, genotyping and metabolic analysis, growth phenotypes under normal and carbon limited conditions, overview Synechocystis sp.
1.4.4.2 additional information inactivation of the sll0171 gene by introducing a spectinomycin-resistance cartridge Synechocystis sp. PCC 6803
1.4.4.2 additional information mutant affected in T-protein subunit of GDC shows similar CO2-dependent regulation as wild-type cells. Glycine decarboxylase knockout mutants exposed to low CO2 accumulate far more glycine and lysine than wild-type cells or mutants with inactivated glycerate pathway Synechocystis sp.
2.1.2.1 additional information SHMT mutant with only partial segregation Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 50000
-
 4 * 50000, Western blot analysis Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.14 glycolate + NAD+ Synechocystis sp. the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview glyoxylate + NADH
-
 ?
1.4.4.2 glycine + H-protein-lipoyllysine Synechocystis sp. PCC 6803
-
 H-protein-S-aminomethyldihydrolipoyllysine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.14 Synechocystis sp.
-
 strain PCC 6803, gene glcD
-
1.4.4.2 Synechocystis sp.
-
 strain PCC 6803
-
1.4.4.2 Synechocystis sp. PCC 6803
-
-
-
2.1.2.1 Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.14 recombinant N-terminally Strep-tagged enzyme from Escherichia coli strain BL21 by streptavidin-tactin affinity chromatography Synechocystis sp.
2.1.2.1 by affinity chromatography Synechocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.14 additional information
-
 determination of internal glycolate concentrations Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.14 glycolate + NAD+
-
 Synechocystis sp. glyoxylate + NADH
-
 ?
1.1.99.14 glycolate + NAD+ the enzyme is involved in the bacterial 2-phosphoglycolate pathway, overview Synechocystis sp. glyoxylate + NADH
-
 ?
1.4.4.2 glycine + H-protein-lipoyllysine
-
 Synechocystis sp. PCC 6803 H-protein-S-aminomethyldihydrolipoyllysine + CO2
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Synechocystis sp. glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.4.2 tetramer T protein, P protein, H protein, L protein Synechocystis sp. PCC 6803
2.1.2.1 tetramer 4 * 50000, Western blot analysis Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.99.14 GlcD
-
 Synechocystis sp.
1.4.4.2 GDC
-
 Synechocystis sp.
1.4.4.2 GDC
-
 Synechocystis sp. PCC 6803
1.4.4.2 Gly decarboxylase complex
-
 Synechocystis sp.
1.4.4.2 glycine decarboxylase
-
 Synechocystis sp.
1.4.4.2 glycine decarboxylase complex
-
 Synechocystis sp. PCC 6803
1.4.4.2 glycine dehydrogenase
-
 Synechocystis sp. PCC 6803
1.4.4.2 T protein
-
 Synechocystis sp.
2.1.2.1 serine hydroxymethyltransferase
-
 Synechocystis sp.
2.1.2.1 SHMT
-
 Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.14 7
-
 assay at Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.14 NAD+
-
 Synechocystis sp.