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Literature summary extracted from

  • O'Brien, M.; Chantha, S.C.; Rahier, A.; Matton, D.P.
    Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity (2005), Plant Physiol., 139, 734-749.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.154 gene CYP51, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Saccharomyces cerevisiae, functional complementation of the DELTAerg11 enzyme deficient yeast mutant strain to 81% cell viability Solanum chacoense

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.154 membrane
-
 Solanum chacoense 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.154 55200
-
 x * 55200, amino acid sequence caculation Solanum chacoense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Solanum chacoense
-
 4,4-dimethylzymosterol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Solanum chacoense the enzyme is involved in biosynthesis of sitosterol and brassinosteroids, pathways overview 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.154 Solanum chacoense Q673E9 gene CYP51
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.154 leaf mature, weak enzyme expression Solanum chacoense
-
1.14.14.154 additional information tissue- and development-specific expression pattern, in situ hybridization, no enzyme expression in stems, petals, stamens, pollen, and in-vitro-grown pollen tubes, overview, enzyme expression is regulated both by pollination and by fertilization Solanum chacoense
-
1.14.14.154 pistil ovules and ovaries without pericarp, transient increase in enzyme expression around 72 h postpollination pistils, weak enzyme expression in unpollinated pistils on the day of anthesis Solanum chacoense
-
1.14.14.154 root weak enzyme expression Solanum chacoense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.154 lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum chacoense 4,4-dimethylzymosterol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum chacoense 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.154 obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in biosynthesis of sitosterol and brassinosteroids, pathways overview Solanum chacoense 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.154 ? x * 55200, amino acid sequence caculation Solanum chacoense

Synonyms

EC Number Synonyms Comment Organism
1.14.14.154 CYP51G1
-
 Solanum chacoense
1.14.14.154 lanosterol demethylase
-
 Solanum chacoense
1.14.14.154 obtusifoliol 14alpha-demethylase
-
 Solanum chacoense
1.14.14.154 sterol 14alpha-demethylase
-
 Solanum chacoense

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.154 Solanum chacoense amino acid sequence caculation
-
 8.7