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Literature summary extracted from

  • Sparla, F.; Zaffagnini, M.; Wedel, N.; Scheibe, R.; Pupillo, P.; Trost, P.
    Regulation of photosynthetic GAPDH dissected by mutants (2005), Plant Physiol., 138, 2210-2219.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.9 into the pET29 vector for expression in Escherichia coli BL21DE3 cells Spinacia oleracea
1.2.1.13 into the pET29 vector for expression in Escherichia coli BL21DE3 cells Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.9 A(plusCTE) chimeric mutant for testing the regulatory function of CTE Spinacia oleracea
1.2.1.9 B(E326Q) site specific mutant of the GAPDH B-subunit Spinacia oleracea
1.2.1.9 B(minCTE) deletion mutant for testing the regulatory function of CTE Spinacia oleracea
1.2.1.9 B(R77A) site specific mutant of the GAPDH B-subunit Spinacia oleracea
1.2.1.9 B(S188A) site specific mutant of the GAPDH B-subunit Spinacia oleracea
1.2.1.13 A(plusCTE) chimeric mutant for testing the regulatory function of CTE Spinacia oleracea
1.2.1.13 B(E326Q) site specific mutant of the GAPDH B-subunit Spinacia oleracea
1.2.1.13 B(minCTE) deletion mutant for testing the regulatory function of CTE Spinacia oleracea
1.2.1.13 B(R77A) site specific mutant of the GAPDH B-subunit Spinacia oleracea
1.2.1.13 B(S188A) site specific mutant of the GAPDH B-subunit Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.9 0.012
-
 3-phospho-D-glyceroyl phosphate mutant B(E362Q) Spinacia oleracea
1.2.1.9 0.015
-
 3-phospho-D-glyceroyl phosphate GapA Spinacia oleracea
1.2.1.9 0.015
-
 3-phospho-D-glyceroyl phosphate GapB, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.016
-
 3-phospho-D-glyceroyl phosphate mutant B(R77A) Spinacia oleracea
1.2.1.9 0.019
-
 3-phospho-D-glyceroyl phosphate A(plusCTE) mutant, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.019
-
 NADPH mutant B(minCTE) Spinacia oleracea
1.2.1.9 0.02
-
 3-phospho-D-glyceroyl phosphate AB-GAPDH, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.02
-
 3-phospho-D-glyceroyl phosphate GapB, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.021
-
 3-phospho-D-glyceroyl phosphate A(plusCTE) mutant, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.022
-
 3-phospho-D-glyceroyl phosphate B(minCTE) mutant Spinacia oleracea
1.2.1.9 0.024
-
 3-phospho-D-glyceroyl phosphate AB-GAPDH, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.024
-
 NADPH mutant B(188)A, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.024
-
 NADPH mutant B(E362Q) Spinacia oleracea
1.2.1.9 0.027
-
 3-phospho-D-glyceroyl phosphate mutant B(188)A, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.029
-
 NADPH GapA Spinacia oleracea
1.2.1.9 0.03
-
 NADPH GapB, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.031
-
 3-phospho-D-glyceroyl phosphate mutant B(188)A, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.038
-
 NADPH GapB, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.043
-
 NADPH A(plusCTE) mutant, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.046
-
 NADPH mutant B(188)A, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.05
-
 NADPH AB-GAPDH, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.9 0.05
-
 NADPH AB-GAPDH, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.065
-
 NADPH A(plusCTE) mutant, analyzed under reducing conditions Spinacia oleracea
1.2.1.9 0.078
-
 NADPH mutant B(R77A) Spinacia oleracea
1.2.1.13 0.012
-
 3-phospho-D-glyceroyl phosphate mutant B(E362Q) Spinacia oleracea
1.2.1.13 0.015
-
 3-phospho-D-glyceroyl phosphate GapA Spinacia oleracea
1.2.1.13 0.015
-
 3-phospho-D-glyceroyl phosphate GapB, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.016
-
 3-phospho-D-glyceroyl phosphate mutant B(R77A) Spinacia oleracea
1.2.1.13 0.019
-
 3-phospho-D-glyceroyl phosphate A(plusCTE) mutant, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.019
-
 NADPH B(minCTE) mutant Spinacia oleracea
1.2.1.13 0.02
-
 3-phospho-D-glyceroyl phosphate AB-GAPDH, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.02
-
 3-phospho-D-glyceroyl phosphate GapB, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.021
-
 3-phospho-D-glyceroyl phosphate A(plusCTE) mutant, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.022
-
 3-phospho-D-glyceroyl phosphate B(minCTE) mutant Spinacia oleracea
1.2.1.13 0.024
-
 3-phospho-D-glyceroyl phosphate AB-GAPDH, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.024
-
 NADPH mutant B(188)A, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.024
-
 NADPH mutant B(E362Q) Spinacia oleracea
1.2.1.13 0.027
-
 3-phospho-D-glyceroyl phosphate mutant B(188)A, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.029
-
 NADPH GapA Spinacia oleracea
1.2.1.13 0.03
-
 NADPH GapB, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.031
-
 3-phospho-D-glyceroyl phosphate mutant B(188)A, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.038
-
 NADPH GapB, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.043
-
 NADPH A(plusCTE) mutant, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.046
-
 NADPH mutant B(188)A, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.05
-
 NADPH AB-GAPDH, analyzed under oxidizing conditions Spinacia oleracea
1.2.1.13 0.05
-
 NADPH AB-GAPDH, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.065
-
 NADPH A(plusCTE) mutant, analyzed under reducing conditions Spinacia oleracea
1.2.1.13 0.078
-
 NADPH mutant B(R77A) Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.9 chloroplast
-
 Spinacia oleracea 9507
-
1.2.1.13 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.9 132000
-
 GapA, estimated by gel filtration Spinacia oleracea
1.2.1.9 151000
-
 B(minCTE) mutant, estimated by gel filtration Spinacia oleracea
1.2.1.9 189000
-
 tetrameric conformation of GAPDH-A2B2 stabilized by NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 232000
-
 GapB mutant B(E326Q) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 243000
-
 wild-type GapB, tetramer in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 247000
-
 GapB mutant B(S188A) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 270000
-
 A(plusCTE) mutant, tetramer in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 278000
-
 GapB mutant B(R77A) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.9 491000
-
 wild-type GapB in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.9 520000
-
 GapB mutant B(E326Q) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.9 520000
-
 GapB mutant B(R77A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.9 553000
-
 GapB mutant B(S188A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.9 760000
-
 oligomer of GAPDH in the presence of NADH, suggesting A8B8 stoichiometry, estimated by gel filtration Spinacia oleracea
1.2.1.13 132000
-
 GapA, estimated by gel filtration Spinacia oleracea
1.2.1.13 151000
-
 B(minCTE) mutant, estimated by gel filtration Spinacia oleracea
1.2.1.13 189000
-
 tetrameric conformation of GAPDH-A2B2 stabilized by NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 232000
-
 GapB mutant B(E326Q) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 243000
-
 wild-type GapB, tetramer in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 247000
-
 GapB mutant B(S188A) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 270000
-
 A(plusCTE) mutant, tetramer in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 278000
-
 GapB mutant B(R77A) in the presence of NADPH, estimated by gel filtration Spinacia oleracea
1.2.1.13 491000
-
 wild-type GapB in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.13 520000
-
 GapB mutant B(E326Q) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.13 520000
-
 GapB mutant B(R77A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.13 553000
-
 GapB mutant B(S188A) in the presence of NADH, compatible with octameric structure, estimated by gel filtration Spinacia oleracea
1.2.1.13 760000
-
 oligomer of GAPDH in the presence of NADH, suggesting A8B8 stoichiometry, estimated by gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.9 3-phospho-D-glyceroyl phosphate + NADPH + H+ Spinacia oleracea
-
 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADPH + H+ Spinacia oleracea
-
 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.9 Spinacia oleracea
-
-
-
1.2.1.13 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.9 using a Q-Sepharose HP and a MonoQ anion-exchange column Spinacia oleracea
1.2.1.13 using a Q-Sepharose HP and a MonoQ anion-exchange column Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.9 leaf
-
 Spinacia oleracea
-
1.2.1.13 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.9 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 Spinacia oleracea D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 Spinacia oleracea D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.9 GAPDH
-
 Spinacia oleracea
1.2.1.9 glyceraldehyde-3-phosphate dehydrogenase
-
 Spinacia oleracea
1.2.1.13 GAPDH
-
 Spinacia oleracea
1.2.1.13 glyceraldehyde-3-phosphate dehydrogenase
-
 Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.9 25
-
 activity assay Spinacia oleracea
1.2.1.13 25
-
 activity assay Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.9 7.9
-
 activity assay Spinacia oleracea
1.2.1.13 7.9
-
 activity assay Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.9 NADPH
-
 Spinacia oleracea
1.2.1.13 NADPH
-
 Spinacia oleracea