EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.71 | 0.0023 | - |
leachianone G | pH 8.8, 30°C | Sophora flavescens | |
2.5.1.71 | 0.059 | - |
dimethylallyl diphosphate | pH 8.8, 30°C | Sophora flavescens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.142 | endoplasmic reticulum | - |
Sophora flavescens | 5783 | - |
1.14.14.142 | additional information | subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview | Sophora flavescens | - |
- |
2.5.1.70 | chloroplast | - |
Sophora flavescens | 9507 | - |
2.5.1.70 | microsome | - |
Sophora flavescens | - |
- |
2.5.1.70 | additional information | subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview | Sophora flavescens | - |
- |
2.5.1.71 | chloroplast | - |
Sophora flavescens | 9507 | - |
2.5.1.71 | membrane | bound, determination of enzyme distibution in membranes | Sophora flavescens | 16020 | - |
2.5.1.71 | additional information | subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview | Sophora flavescens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.70 | Mg2+ | - |
Sophora flavescens | |
2.5.1.71 | Ca2+ | 25% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Co2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Cu2+ | 4% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Fe2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Mg2+ | required, optimal at 10 mM, other divalent cations can only partially substitute for Mg2+, overview | Sophora flavescens | |
2.5.1.71 | Mn2+ | 15% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Ni2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
2.5.1.71 | Zn2+ | 10% of the activity with Mg2+ | Sophora flavescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.142 | 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | Sophora flavescens | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2.5.1.70 | 3,3-dimethylallyl diphosphate + (2S)-naringenin | Sophora flavescens | - |
diphosphate + sophoraflavanone B | - |
? | |
2.5.1.71 | dimethylallyl diphosphate + leachianone G | Sophora flavescens | the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant | diphosphate + sophoraflavanone G | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.142 | Sophora flavescens | - |
- |
- |
2.5.1.70 | Sophora flavescens | - |
- |
- |
2.5.1.71 | Sophora flavescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.142 | native enzyme partially by microsome preparation | Sophora flavescens |
2.5.1.71 | native enzyme partially by microsome preparation | Sophora flavescens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.70 | dimethylallyl diphosphate + (-)-(2S)-naringenin = diphosphate + sophoraflavanone B | reaction mechanism | Sophora flavescens | |
2.5.1.71 | dimethylallyl diphosphate + leachianone G = diphosphate + sophoraflavanone G | reaction mechanism | Sophora flavescens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.142 | cell suspension culture | - |
Sophora flavescens | - |
2.5.1.70 | cell suspension culture | - |
Sophora flavescens | - |
2.5.1.71 | cell suspension culture | - |
Sophora flavescens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.70 | 0.001 | - |
microsomes | Sophora flavescens |
2.5.1.71 | 0.00014 | - |
crude cell extract | Sophora flavescens |
2.5.1.71 | 0.06 | - |
microsomes | Sophora flavescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.142 | 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.142 | 8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview | Sophora flavescens | leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
2.5.1.70 | 3,3-dimethylallyl diphosphate + (2S)-naringenin | - |
Sophora flavescens | diphosphate + sophoraflavanone B | - |
? | |
2.5.1.70 | 3,3-dimethylallyl diphosphate + (2S)-naringenin | i.e. (S)-4',5,7-trihydroxy-flavanone | Sophora flavescens | diphosphate + sophoraflavanone B | - |
? | |
2.5.1.71 | dimethylallyl diphosphate + euchrenone a7 | 54% of the activity with leachianone G | Sophora flavescens | diphosphate + ? | - |
? | |
2.5.1.71 | dimethylallyl diphosphate + leachianone G | the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant | Sophora flavescens | diphosphate + sophoraflavanone G | - |
? | |
2.5.1.71 | dimethylallyl diphosphate + leachianone G | the enzyme is a prenyl side-chain elongation enzyme, the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone, the enzyme is absolutely specific for dimethylallyl diphosphate as prenyl donor and highly specific for leachianone G, the 2'-hydroxy function in leachianone G is indispensable to the activity | Sophora flavescens | diphosphate + sophoraflavanone G | - |
? | |
2.5.1.71 | additional information | no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview | Sophora flavescens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.142 | 8-dimethylallylnaringenin 2'-hydroxylase | - |
Sophora flavescens |
2.5.1.71 | leachianone G 2''-dimethylallyltransferase | - |
Sophora flavescens |
2.5.1.71 | LG 2''-dimethylallyltransferase | - |
Sophora flavescens |
2.5.1.71 | LGDT | - |
Sophora flavescens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.71 | 30 | - |
assay at | Sophora flavescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.71 | 8.8 | - |
- |
Sophora flavescens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.71 | 7.9 | 10 | 50% of maximal activity within this range, almost inactive at pH 6.8, pH profile, overview | Sophora flavescens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.142 | additional information | the enzyme is a cytochrome P450 monooxygenase | Sophora flavescens |