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Literature summary extracted from

  • Ajjawi, I.; Rodriguez Milla, M.A.; Cushman, J.; Shintani, D.K.
    Thiamin pyrophosphokinase is required for thiamin cofactor activation in Arabidopsis (2007), Plant Mol. Biol., 65, 151-162.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.2 isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.6.2 additional information construction of a double knockout mutant lacking isozymes TPK1 and TPK2, the mutant shows severely depressed levels of TPP and elevated levels of free thiamine compared to the wild-type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.2 0.00096
-
 thiamine AtTPK1 Arabidopsis thaliana
2.7.6.2 0.00096
-
 thiamine pH 8.0, 37°C, isozyme TPK1 Arabidopsis thaliana
2.7.6.2 0.00128
-
 thiamine AtTPK2 Arabidopsis thaliana
2.7.6.2 0.00128
-
 thiamine pH 8.0, 37°C, isozyme TPK2 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.2 cytosol
-
 Arabidopsis thaliana 5829
-
2.7.6.2 cytosol isozymes TPK1 and TPK2 Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.2 Mg2+
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.2 ATP + thiamine Arabidopsis thaliana thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis AMP + thiamine diphosphate
-
 ?
2.7.6.2 thiamin + ATP Arabidopsis thaliana
-
 thiamindiphosphate + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Arabidopsis thaliana
-
-
-
2.7.6.2 Arabidopsis thaliana
-
 isozymes TPK1 and TPK2
-
2.7.6.2 Arabidopsis thaliana F4IV16
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.2 flower
-
 Arabidopsis thaliana
-
2.7.6.2 leaf
-
 Arabidopsis thaliana
-
2.7.6.2 additional information isozymes TPK1 and TPK2 are biochemically redundant cytosolic proteins that are similarly expressed throughout different plant tissues Arabidopsis thaliana
-
2.7.6.2 root
-
 Arabidopsis thaliana
-
2.7.6.2 silique
-
 Arabidopsis thaliana
-
2.7.6.2 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 ATP + thiamine thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis Arabidopsis thaliana AMP + thiamine diphosphate
-
 ?
2.7.6.2 ATP + thiamine no TMP kinase activity is shown by either isozymes TPK1 or TPK2 Arabidopsis thaliana AMP + thiamine diphosphate
-
 ?
2.7.6.2 thiamin + ATP
-
 Arabidopsis thaliana thiamindiphosphate + AMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.6.2 At1g02880 (AtTPK1)
-
 Arabidopsis thaliana
2.7.6.2 At2g44750 (AtTPK2)
-
 Arabidopsis thaliana
2.7.6.2 thiamin pyrophosphokinase
-
 Arabidopsis thaliana
2.7.6.2 TPK
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.2 37
-
 AtTPK2 Arabidopsis thaliana
2.7.6.2 37
-
 isozyme TPK2 Arabidopsis thaliana
2.7.6.2 45
-
 AtTPK1 Arabidopsis thaliana
2.7.6.2 45
-
 isozyme TPK1 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.6.2 37 50 37°C: optimal activity of TPK2, 50°C: TPK2 is completely inactive Arabidopsis thaliana
2.7.6.2 45 55 TPK1 Arabidopsis thaliana
2.7.6.2 50
-
 AtTPK2 is completely inactive Arabidopsis thaliana
2.7.6.2 55
-
 AtTPK1 is still active Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.2 50
-
 inactivation of isozyme TPK2 Arabidopsis thaliana
2.7.6.2 55
-
 isozyme TPK1 shows remaining activity Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.2 7
-
-
 Arabidopsis thaliana
2.7.6.2 7
-
 isozymes TPK1 or TPK2 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.2 6 8
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.6.2 ATP
-
 Arabidopsis thaliana