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Literature summary extracted from

  • Islam, M.R.; Kim, H.; Kang, S.W.; Kim, J.S.; Jeong, Y.M.; Hwang, H.J.; Lee, S.Y.; Woo, J.C.; Kim, S.G.
    Functional characterization of a gene encoding a dual domain for uridine kinase and uracil phosphoribosyltransferase in Arabidopsis thaliana (2007), Plant Mol. Biol., 63, 465-477.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview Arabidopsis thaliana
2.4.2.9 expressed in Escherichia coli strain JM109 Arabidopsis thaliana
2.7.1.48
-
 Arabidopsis thaliana
2.7.1.48 expressed in Escherichia coli strain JM109 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.48 additional information contrary to wild-type, enzyme T-DNA insertion mutant plants are not affected in growth when treated with 5-fluorouracil or 5-fluorouridine. Expression of UK/UPRT1 in upp and uppudk mutants of Escherichia coli supplied with 5-fluorouracil and 5-fluorouridine leads to growth inhibition. Identical results are obtained with 5-fluorouridine and 5-fluorouracil treatments when the uridine kinase and uracil phosphoribosyltransferase domains are separated by the introduction of translation initiation and stop codons prior to complementation into the upp-udk and upp mutants Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.9 cytoplasm forming granular precipitates Arabidopsis thaliana 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 53000
-
 x * 53000, recombinant UK/UPRT1, SDS-PAGE Arabidopsis thaliana
2.7.1.48 53000
-
 SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate Arabidopsis thaliana recombinant bifunctional enzyme UK/UPRT 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Arabidopsis thaliana
-
 UMP + diphosphate
-
 ?
2.4.2.9 uridine + ATP Arabidopsis thaliana the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 UMP + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Arabidopsis thaliana Q9FKS0 ecotype Col-0
-
2.4.2.9 Arabidopsis thaliana Q9FKS0 AtUK/UPRT1; ecotype Col-0, several strains, natural chimeric gene AtUK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT
-
2.7.1.48 Arabidopsis thaliana Q9FKS0 bifunctional uridine kinase/uracil phosphoribosyltransferase UK/UPRT1
-
2.7.1.48 Arabidopsis thaliana Q9FKS0 uridine kinase-like protein; ecotype Col-0
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.9 flower
-
 Arabidopsis thaliana
-
2.4.2.9 flower lower expression level Arabidopsis thaliana
-
2.4.2.9 leaf
-
 Arabidopsis thaliana
-
2.4.2.9 leaf higher expression level Arabidopsis thaliana
-
2.4.2.9 additional information ubiquitous expression of UK/UPRT Arabidopsis thaliana
-
2.4.2.9 root
-
 Arabidopsis thaliana
-
2.4.2.9 root lower expression level Arabidopsis thaliana
-
2.4.2.9 stem
-
 Arabidopsis thaliana
-
2.4.2.9 stem higher expression level Arabidopsis thaliana
-
2.7.1.48 flower
-
 Arabidopsis thaliana
-
2.7.1.48 leaf
-
 Arabidopsis thaliana
-
2.7.1.48 root
-
 Arabidopsis thaliana
-
2.7.1.48 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate recombinant bifunctional enzyme UK/UPRT Arabidopsis thaliana 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Arabidopsis thaliana UMP + diphosphate
-
 ?
2.4.2.9 uridine + ATP the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 Arabidopsis thaliana UMP + ADP
-
 ?
2.7.1.48 ATP + 5-fluorouridine
-
 Arabidopsis thaliana ADP + 5-fluoro-uridine monophosphate
-
 ?
2.7.1.48 ATP + uridine
-
 Arabidopsis thaliana ADP + UMP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 ? x * 53000, recombinant UK/UPRT1, SDS-PAGE Arabidopsis thaliana
2.4.2.9 More the recombinant chimeric enzyme UK/UPRT1 shows an N-terminal region containing a UK domain and the C-terminal region consisting of an UPRT domain Arabidopsis thaliana
2.7.1.48 ? x * 53000, calculated Arabidopsis thaliana
2.7.1.48 More N-terminal region of UK/UPRT1 contains an uridine kinase domain, and the C-terminal region consists of an uracil phosphoribosyltransferase domain Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 More cf. EC 2.7.1.48 Arabidopsis thaliana
2.4.2.9 UK/UPRT1
-
 Arabidopsis thaliana
2.4.2.9 UPRT
-
 Arabidopsis thaliana
2.4.2.9 uridine kinase-like protein (uracil phosphoribosyltransferase)
-
 Arabidopsis thaliana
2.4.2.9 uridine kinase/uracil phosphoribosyltransferase 1 the enzyme contains a uridine domain and the C-terminal region consists of a UPRT domain Arabidopsis thaliana
2.7.1.48 UK/UPRT1 double catalytic enzyme for uridine kinase (N-terminal domain) and uracil phosphoribosyltransferase (C-terminal domain) Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.48 ATP
-
 Arabidopsis thaliana