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Literature summary extracted from

  • Li, H.M.; Rotter, D.; Hartman, T.G.; Pak, F.E.; Havkin-Frenkel, D.; Belanger, F.C.
    Evolution of novel O-methyltransferases from the Vanilla planifolia caffeic acid O-methyltransferase (2006), Plant Mol. Biol., 61, 537-552.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.68 additional information Vanilla planifolia OMT-2 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences Vanilla planifolia
2.1.1.68 additional information Vanilla planifolia OMT-3 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences Vanilla planifolia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.68 expression as N-terminal polyhistidine-tagged fusion in Escherichia coli BL21-CodonPlus (DE3)-RIPL competent cells based on the expression vector pET-15bexpression Vanilla planifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.114
-
myricetin
-
Vanilla planifolia
2.1.1.68 0.96
-
methyl gallate
-
Vanilla planifolia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 38770
-
sequence analysis Vanilla planifolia
2.1.1.68 38920
-
sequence analysis Vanilla planifolia

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Vanilla planifolia Q27I62
-
-
2.1.1.68 Vanilla planifolia Q27I63
-
-
2.1.1.68 Vanilla pompona
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 by nickel affinity chromatography Vanilla planifolia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 flower
-
Vanilla planifolia
-
2.1.1.68 additional information no activity in stem, leaf and bean Vanilla planifolia
-
2.1.1.68 root
-
Vanilla pompona
-
2.1.1.68 root most highly expressed Vanilla planifolia
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.68 -80°C, Tris-HCl buffer, pH 7.5 Vanilla planifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 delphinidin
-
Vanilla planifolia ?
-
?
2.1.1.68 ethyl gallate
-
Vanilla planifolia ?
-
?
2.1.1.68 methyl gallate
-
Vanilla planifolia ?
-
?
2.1.1.68 additional information does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin Vanilla planifolia ?
-
?
2.1.1.68 myricetin
-
Vanilla planifolia ?
-
?
2.1.1.68 myricitrin
-
Vanilla planifolia ?
-
?
2.1.1.68 n-propyl gallate best substrate Vanilla planifolia ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.68 caffeic acid O-methyltransferase
-
Vanilla planifolia
2.1.1.68 caffeic acid O-methyltransferase
-
Vanilla pompona
2.1.1.68 COMT
-
Vanilla planifolia
2.1.1.68 COMT
-
Vanilla pompona
2.1.1.68 Van OMT-2
-
Vanilla planifolia
2.1.1.68 Van OMT-2
-
Vanilla pompona
2.1.1.68 Van OMT-3
-
Vanilla planifolia
2.1.1.68 Van OMT-3
-
Vanilla pompona

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.68 0.068
-
myricetin
-
Vanilla planifolia
2.1.1.68 0.23
-
methyl gallate
-
Vanilla planifolia