EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.68 | additional information | Vanilla planifolia OMT-2 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences | Vanilla planifolia |
2.1.1.68 | additional information | Vanilla planifolia OMT-3 is most similar to caffeic acid O-methyltransferases, reveals new instance of COMT-like enzyme with novel substrate preferences | Vanilla planifolia |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.68 | expression as N-terminal polyhistidine-tagged fusion in Escherichia coli BL21-CodonPlus (DE3)-RIPL competent cells based on the expression vector pET-15bexpression | Vanilla planifolia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.68 | 0.114 | - |
myricetin | - |
Vanilla planifolia | |
2.1.1.68 | 0.96 | - |
methyl gallate | - |
Vanilla planifolia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.68 | 38770 | - |
sequence analysis | Vanilla planifolia |
2.1.1.68 | 38920 | - |
sequence analysis | Vanilla planifolia |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.68 | Vanilla planifolia | Q27I62 | - |
- |
2.1.1.68 | Vanilla planifolia | Q27I63 | - |
- |
2.1.1.68 | Vanilla pompona | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.68 | by nickel affinity chromatography | Vanilla planifolia |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.68 | flower | - |
Vanilla planifolia | - |
2.1.1.68 | additional information | no activity in stem, leaf and bean | Vanilla planifolia | - |
2.1.1.68 | root | - |
Vanilla pompona | - |
2.1.1.68 | root | most highly expressed | Vanilla planifolia | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.68 | -80°C, Tris-HCl buffer, pH 7.5 | Vanilla planifolia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.68 | delphinidin | - |
Vanilla planifolia | ? | - |
? | |
2.1.1.68 | ethyl gallate | - |
Vanilla planifolia | ? | - |
? | |
2.1.1.68 | methyl gallate | - |
Vanilla planifolia | ? | - |
? | |
2.1.1.68 | additional information | does not have significant activity with the typical COMT substrates, caffeoyl aldehyde, 5-OH-coniferaldehyde, 5-OH-ferulic acid or caffeic acid or with the proposed vanillin precursor 3,4-dihydroxybenzaldehyde, thus is no component of the vanillin biosynthetic pathway, also no activity with kaempferol or quercetin | Vanilla planifolia | ? | - |
? | |
2.1.1.68 | myricetin | - |
Vanilla planifolia | ? | - |
? | |
2.1.1.68 | myricitrin | - |
Vanilla planifolia | ? | - |
? | |
2.1.1.68 | n-propyl gallate | best substrate | Vanilla planifolia | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.68 | caffeic acid O-methyltransferase | - |
Vanilla planifolia |
2.1.1.68 | caffeic acid O-methyltransferase | - |
Vanilla pompona |
2.1.1.68 | COMT | - |
Vanilla planifolia |
2.1.1.68 | COMT | - |
Vanilla pompona |
2.1.1.68 | Van OMT-2 | - |
Vanilla planifolia |
2.1.1.68 | Van OMT-2 | - |
Vanilla pompona |
2.1.1.68 | Van OMT-3 | - |
Vanilla planifolia |
2.1.1.68 | Van OMT-3 | - |
Vanilla pompona |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.68 | 0.068 | - |
myricetin | - |
Vanilla planifolia | |
2.1.1.68 | 0.23 | - |
methyl gallate | - |
Vanilla planifolia |