BRENDA - Enzyme Database

The Chlamydomonas reinhardtii gtr gene encoding the tetrapyrrole biosynthetic enzyme glutamyl-trna reductase: structure of the gene and properties of the expressed enzyme

Srivastava, A.; Lake, V.; Nogaj, L.A.; Mayer, S.M.; Willows, R.D.; Beale, S.I.; Plant Mol. Biol. 58, 643-658 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
expression as His-tag fusion protein in Escherichia coli
Chlamydomonas reinhardtii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
heme
80% inhibition at 0.005 mM
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
52500
-
deduced from sequence
Chlamydomonas reinhardtii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Chlamydomonas reinhardtii
Q9FPR7
-
-
1.2.1.70
Chlamydomonas reinhardtii CC-124
Q9FPR7
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
Ni-affinity chromatography
Chlamydomonas reinhardtii
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.70
cell culture
-
Chlamydomonas reinhardtii
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADH + H+
the enzyme also exhibits an esterase activity
676535
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NAD+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
homodimer
2 * 52500, gel filtration
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADH
-
Chlamydomonas reinhardtii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
expression as His-tag fusion protein in Escherichia coli
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADH
-
Chlamydomonas reinhardtii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
heme
80% inhibition at 0.005 mM
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
52500
-
deduced from sequence
Chlamydomonas reinhardtii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
Ni-affinity chromatography
Chlamydomonas reinhardtii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.70
cell culture
-
Chlamydomonas reinhardtii
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADH + H+
the enzyme also exhibits an esterase activity
676535
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NAD+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
homodimer
2 * 52500, gel filtration
Chlamydomonas reinhardtii