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Literature summary extracted from

  • Sinnige, M.P.; Roobeek, I.; Bunney, T.D.; Visser, A.J.; Mol, J.N.; de Boer, A.H.
    Single amino acid variation in barley 14-3-3 proteins leads to functional isoform specificity in the regulation of nitrate reductase (2005), Plant J., 44, 1001-1009.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 14-3-3B protein
-
 Hordeum vulgare
1.7.1.1 additional information the 14-3-3A protein and the 14-3-3C protein are functionally not capable to inhibit nitrate reductase Hordeum vulgare
1.7.1.1 mutant G216S of 14-3-3A protein
-
 Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1 HiTrap Q column anion-exchange chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.1 leaf
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 nitrate + NADH
-
 Hordeum vulgare nitrite + NAD+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Hordeum vulgare

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.1 0.0000015
-
 14-3-3B protein in the presence of 5 mM Mg2+ Hordeum vulgare
1.7.1.1 0.0000017
-
 14-3-3B protein in the presence of 5 mM Ca2+ Hordeum vulgare
1.7.1.1 0.0000049
-
 mutant G216S of 14-3-3A protein in the presence of 5 mM Ca2+ Hordeum vulgare
1.7.1.1 0.0000054
-
 mutant G216S of 14-3-3A protein in the presence of 5 mM Mg2+ Hordeum vulgare